Antioxidant peptides derived from the hydrolyzate of purple sea urchin (Strongylocentrotus nudus) gonad alleviate oxidative stress in Caenorhabditis elegans
| Autor: | Chung Wah Ma, Sixu Zhao, Weizhang Jia, Qiong Cheng, Ming Liang, Xiquan Yin, Qiong Peng, Zebo Huang, Xuesong Yu |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Antioxidant Antioxidant peptide medicine.medical_treatment Medicine (miscellaneous) Peptide medicine.disease_cause 03 medical and health sciences chemistry.chemical_compound RNA interference biology.animal medicine Purple sea urchin Strongylocentrotus nudus TX341-641 Caenorhabditis elegans Sea urchin chemistry.chemical_classification Reactive oxygen species Nutrition and Dietetics 030102 biochemistry & molecular biology biology Nutrition. Foods and food supply Superoxide biology.organism_classification 030104 developmental biology Biochemistry chemistry Oxidative stress Food Science |
| Zdroj: | Journal of Functional Foods, Vol 48, Iss, Pp 594-604 (2018) |
| ISSN: | 1756-4646 |
| DOI: | 10.1016/j.jff.2018.07.060 |
| Popis: | The papain hydrolyzate prepared from purple sea urchin (Strongylocentrotus nudus) gonad exerts significant antioxidant activity. Through ultrafiltration, gel filtration and high performance liquid chromatography, the active fractions from the hydrolyzate were screened by alleviating paraquat-induced oxidative stress in Caenorhabditis elegans, and the peptide components were identified by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Two antioxidant peptides SnP7 (AAVPSGASTGIYEALELR, 1805.03 Da) and SnP10 (NPLLEAFGNAK, 1173.34 Da) exhibit significant antioxidant capacity, which reduce reactive oxygen species (ROS) level and the expression of superoxide dismutase-3 (SOD-3) and heat shock protein-16.2 (HSP-16.2) in oxidation-damaged nematodes. Further studies indicated that SnP7 and SnP10 induce DAF-16 nuclear translocation and the expression of stress-related genes such as sod-3. RNA interference (RNAi) suggested that peptide-induced enhancement of resistance is dependent on DAF-16. Taken together, S. nudus antioxidant peptides might act through activate DAF-16 signaling pathway, and induce the expression of DAF-16 target genes that enhance antioxidant potential of organisms. |
| Databáze: | OpenAIRE |
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