A two-dimensional thin-layer chromatography procedure for simultaneous separation of ceramide and diacylglycerol species
| Autor: | O Koul, M. El Babili, Georges Zwingelstein, Gérard Brichon, Jacques Bodennec |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Gills
Ceramide Analytical chemistry Myristic acid QD415-436 Ceramides Tritium Myristic Acid Biochemistry Diglycerides chemistry.chemical_compound Endocrinology Animals Sea bass Diacylglycerol kinase Solvent system Chromatography Chemistry Silica gel Cell Biology Thin-layer chromatography Evaluation Studies as Topic Bass lipids (amino acids peptides and proteins) Chromatography Thin Layer |
| Zdroj: | Journal of Lipid Research, Vol 38, Iss 8, Pp 1702-1706 (1997) |
| ISSN: | 0022-2275 |
| DOI: | 10.1016/s0022-2275(20)37188-1 |
| Popis: | We have developed a novel, simple, and rapid, two-dimensional thin-layer chromatography method to separate 1,2-, 1,3-diacylglycerols and ceramides containing alpha-hydroxy and normal fatty acids from other neutral lipids on one 10 x 10 cm precoated silica gel plate. The three solvent systems used in succession leave the phospholipids at the origin and separate neutral lipids of interest into component species. We have applied this method to incorporation of 9,10-[3H]myristic acid into lipids of gills from sea bass and obtained results that are similar and comparable to those obtained by described methods. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|