| Autor: |
Juxiang Liu, Ping Chen, Xulong Hu, Liping Huang, Zhi Geng, Hao Xu, Wenjun Hu, Lin Wang, Ping Wu, Gang L. Liu |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Chemical engineering journal (Lausanne, Switzerland : 1996). 451 |
| ISSN: |
1385-8947 |
| Popis: |
The novel mutations attributed by the high mutagenicity of the SARS-CoV-2 makes its prevention and treatment challenging. Developing an ultra-fast, point-of-care-test (POCT) protocol is critical for responding to large-scale spread of SARS-CoV-2 in public places and in resource-poor remote areas. Here, we developed a nanoplasmonic enhanced isothermal amplification (NanoPEIA) strategy that combines a nanoplasmonic sensor with isothermal amplification. The novel strategy provides an ideal easy-to operate detection platform for obtaining accurate, ultra-fast and high-throughput (96 samples can be tested together) data. For clinical samples with viral detection at Ct value25, the entire process (including sample preparation, virus lysis, detection, and data analysis) can be completed within six minutes. The method is also appropriate for detection of SARS-CoV-2 |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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