Crystal structure of a nucleocapsid-like nucleoprotein-RNA complex of respiratory syncytial virus
| Autor: | Clemens Vonrhein, Nathalie Castagné, Rajiv G. Tawar, David Bhella, Laurence Damier-Piolle, Félix A. Rey, Hugues Bedouelle, Kirsty MacLellan, Gérard Bricogne, Stéphane Duquerroy, Paloma F. Varela, Jean-François Eléouët |
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| Přispěvatelé: | Virologie Structurale, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Université Paris-Sud - Paris 11 (UP11), Global Phasing, Unité de recherche Virologie et Immunologie Moléculaires (VIM), Institut National de la Recherche Agronomique (INRA), Medical Research Council Virology Unit, University of Glasgow, Prévention et Thérapie Moléculaires des Maladies Infectieuses Humaines, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Unité de recherche Virologie et Immunologie Moléculaires (VIM (UR 0892)) |
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Models
Molecular Secondary Image Processing [SDV]Life Sciences [q-bio] viruses MESH: Protein Structure Secondary MESH: Amino Acid Sequence Crystallography X-Ray Protein Structure Secondary MESH: Protein Structure Tertiary Computer-Assisted Models Image Processing Computer-Assisted Viral Mononegavirales Polymerase 0303 health sciences Crystallography Multidisciplinary biology Nucleocapsid Proteins MESH: Protein Subunits MESH: Image Processing Computer-Assisted Respiratory Syncytial Viruses 3. Good health MESH: Nucleic Acid Conformation MESH: RNA Viral RNA Viral MESH: Cryoelectron Microscopy MESH: Models Molecular Protein Structure Paramyxoviridae Molecular Sequence Data MESH: Respiratory Syncytial Viruses RNA-dependent RNA polymerase Virus 03 medical and health sciences Amino Acid Sequence 030304 developmental biology Binding Sites MESH: Molecular Sequence Data 030306 microbiology Cryoelectron Microscopy Molecular RNA RNA virus MESH: Nucleocapsid Proteins biology.organism_classification MESH: Crystallography X-Ray Virology Protein Structure Tertiary Nucleoprotein Protein Subunits MESH: Binding Sites X-Ray biology.protein Nucleic Acid Conformation Tertiary |
| Zdroj: | Science Science, American Association for the Advancement of Science, 2009, 326 (5957), pp.1279-83. ⟨10.1126/science.1177634⟩ Science, 2009, 326 (5957), pp.1279-83. ⟨10.1126/science.1177634⟩ |
| ISSN: | 0036-8075 1095-9203 |
| DOI: | 10.1126/science.1177634⟩ |
| Popis: | RSV in 3D Respiratory syncytial virus (RSV) causes pneumonia and bronchiolitis in infants. RSV is an RNA virus in which the genomic RNA forms part of a nuclease-resistant helical ribonucleoprotein complex. Tawar et al. (p. 1279 ) now use x-ray and electron microscopy data to model the structure of this nucleocapsid complex and show how it can template RNA synthesis. The crystal structure shows RNA wrapped around a decameric ring of nucleocapsid protein. Combining this structure with electron microscopy data gives a model that shows how polymerase might read out the RNA bases without disassembling the nucleocapsid helix. |
| Databáze: | OpenAIRE |
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