Polyomavirus infections and its clinical relevance in cancer patients: A Prospective Study
| Autor: | Entsar A. El-Moniem Ahmed, Manar M. Moneer, El-Chaimaa B. Mohamed, Samah A. Loutfy, Emad Abada, Lamiaa H. Ibrahim, Salem E. Salem |
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| Jazyk: | angličtina |
| Předmět: |
Male
0301 basic medicine Polymerase Chain Reaction Gastroenterology law.invention Pathogenesis Hematological malignancies 0302 clinical medicine law Neoplasms Prevalence Overall survival Prospective Studies 030212 general & internal medicine Prospective cohort study Polymerase chain reaction Leukopenia lcsh:Public aspects of medicine General Medicine Middle Aged Viral Load JC Virus BK and JC polyomavirus infections Infectious Diseases Female medicine.symptom Adult medicine.medical_specialty Adolescent Real-Time Polymerase Chain Reaction lcsh:Infectious and parasitic diseases Young Adult 03 medical and health sciences Internal medicine medicine Humans lcsh:RC109-216 Clinical significance Aged Polyomavirus Infections business.industry Public Health Environmental and Occupational Health Cancer lcsh:RA1-1270 biochemical phenomena metabolism and nutrition medicine.disease Survival Analysis Lymphoma 030104 developmental biology BK Virus DNA Viral Immunology business Real-time PCR |
| Zdroj: | Journal of Infection and Public Health, Vol 10, Iss 1, Pp 22-30 (2017) |
| ISSN: | 1876-0341 |
| DOI: | 10.1016/j.jiph.2016.01.008 |
| Popis: | Summary: BK and JC polyomaviruses (PyV) have been demonstrated to be associated with the pathogenesis of various human cancers. We aimed to investigate the impact of BK and JC polyomavirus infections on several clinical parameters in different human cancers. A total of 150 cancer patients were included in the study (51 patients with solid tumors, 48 patients with lymphomas and 51 patients with leukemias). Amplification of PyV DNA was performed using a semi-nested version of Polymerase chain reaction targeting the T genomic region of PyV. The polyomavirus load was determined using real-time PCR assay. The clinical data were collected. Polyomavirus DNA could be detected in 84 (56%) of 150 of all cancerous patients. The solid tumors had the lowest proportion of JCV (6 (11.8%) of 51), whereas had the highest proportion of JCV (200 copies/μl). JCV was more frequent among NHL patients (30%) and absent in HL patients (0%). During follow-up, PyV positivity decreased significantly (p = 0.004) in lymphoma patients (n = 28). Although PyV positivity decreased significantly from 39% to 7% in 28 of 48 lymphoma patients after treatment, it significantly persisted in leukemic patients after treatment (from 22% to 38%). JC was more frequent among leukemic patients with leukopenia. The presence of JC polyomavirus was more frequent among leukemic patients without any significant impact on their overall survival. Keywords: BK and JC polyomavirus infections, Real-time PCR, Hematological malignancies, Overall survival |
| Databáze: | OpenAIRE |
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