Sequence-specific DNA binding by Ku autoantigen and its effects on transcription
| Autor: | Heather Torrance, Ward Giffin, Louise Pope, Robert J. G. Haché, David J. Rodda, Gratien G. Prefontaine |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Ku80
Transcription Genetic HMG-box Molecular Sequence Data DNA-Activated Protein Kinase Mice SCID Protein Serine-Threonine Kinases Biology Autoantigens Cell Line Mice Cricetulus Cricetinae Sequence-specific DNA binding Animals Phosphorylation Ku Autoantigen Repetitive Sequences Nucleic Acid Multidisciplinary Base Sequence DNA Helicases DNA replication Nuclear Proteins Antigens Nuclear DNA-binding domain Molecular biology Ku Protein DNA-Binding Proteins DNA binding site Mammary Tumor Virus Mouse DNA Viral DNA polymerase mu Plasmids Protein Binding Transcription Factors |
| Zdroj: | Nature. 380:265-268 |
| ISSN: | 1476-4687 0028-0836 |
| DOI: | 10.1038/380265a0 |
| Popis: | DNA-dependent protein kinase (DNA-PK) has been implicated in several nuclear processes including transcription, DNA replication, double-stranded DNA break repair, and V(D)J recombination. Linkage of kinase and substrate on DNA in cis is required for efficient phosphorylation. Recruitment of DNA-PK to DNA is by Ku autoantigen, a DNA-end-binding protein required for DNA-PK catalytic activity. Although Ku is known to translocate along naked DNA, how DNA-end binding by Ku might lead to DNA-PK-mediated phosphorylation of sequence-specific DNA-binding proteins in vivo has not been obvious. Here we report the identification of Ku as a transcription factor that recruits DNA-PK directly to specific DNA sequences. NRE1 (negative regulatory element 1) is a DNA sequence element (-394/ -381) in the long terminal repeat of mouse mammary tumour virus (MMTV) that is important for repressing inappropriate viral expression. We show that direct binding of Ku/DNA-PK to NRE1 represses glucocorticoid-induced MMTV transcription. |
| Databáze: | OpenAIRE |
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