The Podospora anserina lytic polysaccharide monooxygenase PaLPMO9H catalyzes oxidative cleavage of diverse plant cell wall matrix glycans

Autor: Jean-Guy Berrin, Harry Brumer, Mathieu Fanuel, Sona Garajova, Nicholas McGregor, David Ropartz, Hélène Rogniaux
Přispěvatelé: Unité de recherche sur les Biopolymères, Interactions Assemblages (BIA), Institut National de la Recherche Agronomique (INRA), Biodiversité et Biotechnologie Fongiques (BBF), Institut National de la Recherche Agronomique (INRA)-Aix Marseille Université (AMU)-École Centrale de Marseille (ECM), Michael Smith Laboratories, University of British Columbia (UBC), Department of Chemistry [Vancouver] (UBC Chemistry), Department of Biochemistry, Department of Botany [Vancouver] (UBC Botany), AMIDEX foundation (Funcopper project) A*M-AAP-EI-13-13-130115-15.37 / MicrobioE project ANR-11-IDEX-0001-02, École Centrale de Marseille (ECM)-Aix Marseille Université (AMU)-Institut National de la Recherche Agronomique (INRA), Department of Chemistry [Vancouver], Department of Botany, Aix Marseille Université (AMU)-Institut National de la Recherche Agronomique (INRA)-École Centrale de Marseille (ECM)
Jazyk: angličtina
Rok vydání: 2017
Předmět:
0106 biological sciences
0301 basic medicine
[SDV.BIO]Life Sciences [q-bio]/Biotechnology
mass spectrum analysis
matrice extracellulaire
Glucomannan
Biotechnologies
Management
Monitoring
Policy and Law
tyrosinase
Polysaccharide
Cleavage (embryo)
01 natural sciences
Applied Microbiology and Biotechnology
Podospora anserina
Cell wall
03 medical and health sciences
chemistry.chemical_compound
métabolisme oxydatif
lignocellulose
podospora anserina
Polysaccharides
AA9
spectrométrie de masse
010608 biotechnology
biomasse
Biomass
LPMO
Cellulose
2. Zero hunger
chemistry.chemical_classification
biology
Mass spectrometry
Renewable Energy
Sustainability and the Environment
Glycosidic bond
biology.organism_classification
Biorefinery
Xyloglucan
030104 developmental biology
General Energy
chemistry
Biochemistry
polysaccharide
Lignocellulose
Biotechnology
oxydoréductase
Zdroj: Biotechnology for Biofuels
Biotechnology for Biofuels, BioMed Central, 2017, 10 (1), ⟨10.1186/s13068-017-0749-5⟩
Biotechnology for Biofuels, 2017, 10 (1), ⟨10.1186/s13068-017-0749-5⟩
Biotechnology for Biofuels 1 (10), . (2017)
ISSN: 1754-6834
Popis: The enzymatic conversion of plant biomass has been recently revolutionized by the discovery of lytic polysaccharide monooxygenases (LPMO) that catalyze oxidative cleavage of polysaccharides. These powerful enzymes are secreted by a large number of fungal saprotrophs and are important components of commercial enzyme cocktails used for industrial biomass conversion. Among the 33 AA9 LPMOs encoded by the genome of Podospora anserina, the PaLPMO9H enzyme catalyzes mixed C1/C4 oxidative cleavage of cellulose and cello-oligosaccharides. Activity of PaLPMO9H on several hemicelluloses has been suggested, but the regioselectivity of the cleavage remained to be determined. In this study, we investigated the activity of PaLPMO9H on mixed-linkage glucans, xyloglucan and glucomannan using tandem mass spectrometry and ion mobility–mass spectrometry. Structural analysis of the released products revealed that PaLPMO9H catalyzes C4 oxidative cleavage of mixed-linkage glucans and mixed C1/C4 oxidative cleavage of glucomannan and xyloglucan. Gem-diols and ketones were produced at the non-reducing end, while aldonic acids were produced at the reducing extremity of the products. The ability of PaLPMO9H to target polysaccharides, differing from cellulose by their linkages, glycosidic composition and/or presence of sidechains, could be advantageous for this coprophilous fungus when catabolizing highly variable polysaccharides and for the development of optimized enzyme cocktails in biorefineries.
Databáze: OpenAIRE
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