Stimulatory effects of polar glycopeptidolipids of Mycobacterium chelonae on murine haematopoietic stem cells and megakaryocyte progenitors
| Autor: | C. Pilet, S. Vincent-Naulleau, D. Thibault, T. Neway |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Immunology
Spleen Bone Marrow Cells Biology Colony-Forming Units Assay Mice Megakaryocyte Adjuvants Immunologic Bone Marrow medicine Cytotoxic T cell Animals Progenitor cell Megakaryocytopoiesis Bone Marrow Transplantation Mice Inbred BALB C Mycobacterium chelonae Hematopoietic Stem Cells Molecular biology Hematopoiesis Haematopoiesis Transplantation Isogeneic medicine.anatomical_structure Female Bone marrow Stem cell Glycoconjugates Megakaryocytes |
| Zdroj: | Research in immunology. 148(2) |
| ISSN: | 0923-2494 |
| Popis: | Summary The effects of polar glycopeptidolipids of Mycobacterium chelonae (pGPL-Mc) on haematopoietic stem cells and on megakaryocyte progenitors in bone marrow (BM) and spleen were investigated in mice. We studied the in vivo spleen colony-forming ability and marrow repopulating ability of pGPL-Mc by assays of colony-forming units-spleen (CFU-S). The number of CFU-S was increased in BM when both donors and recipients were treated with pGPL-Mc. In contrast, a single treatment of donors induced enhancement of spleen CFU-S. The number of pre-CFU-S was not significantly increased by pGPL-Mc injection. Megakaryocyte (Meg) progenitors were determined in vitro with a quantitative cultural analysis of bone marrow and spleen cells in agar in the presence of spleen-conditioned medium. A statistically significant increase in BM and spleen CFU-Meg was observed two days after the last administration of pGPL-Mc. This experiment points out the ability of pGPL-Mc to induce substantial stimulation of megakaryocytopoiesis and slight proliferation of stem cells in BM, but which is more pronounced in spleen. This molecule therefore appears to be a potential adjuvant of chemo- and radiotherapy in order to palliate the cytotoxic side effects of these cancer therapeutic modalities. |
| Databáze: | OpenAIRE |
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