| Autor: |
Yvonne Kohl, Nicola William, Elisabeth Elje, Nadine Backes, Mario Rothbauer, Annamaria Srancikova, Elise Rundén-Pran, Naouale El Yamani, Rafi Korenstein, Lea Madi, Alexander Barbul, Katarina Kozics, Monika Sramkova, Karen Steenson, Alena Gabelova, Peter Ertl, Maria Dusinska, Andrew Nelson |
| Přispěvatelé: |
Publica |
| Jazyk: |
angličtina |
| Rok vydání: |
2023 |
| Předmět: |
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| Zdroj: |
Bioelectrochemistry |
| ISSN: |
1567-5394 |
| Popis: |
This study compares the performance and output of an electrochemical phospholipid membrane platform againstrespective in vitro cell-based toxicity testing methods using three toxicants of different biological action(chlorpromazine (CPZ), colchicine (COL) and methyl methanesulphonate (MMS)). Human cell lines from sevendifferent tissues (lung, liver, kidney, placenta, intestine, immune system) were used to validate this physicochemicaltesting system. For the cell-based systems, the effective concentration at 50 % cell death (EC50) valuesare calculated. For the membrane sensor, a limit of detection (LoD) value was extracted as a quantitativeparameter describing the minimum concentration of toxicant which significantly affects the structure of thephospholipid sensor membrane layer. LoD values were found to align well with the EC50 values when acute cellviability was used as an end-point and showed a similar toxicity ranking of the tested toxicants. Using the colonyforming efficiency (CFE) or DNA damage as end-point, a different order of toxicity ranking was observed. Theresults of this study showed that the electrochemical membrane sensor generates a parameter relating to biomembranedamage, which is the predominant factor in decreasing cell viability when in vitro models are acutelyexposed to toxicants. These results lead the way to using electrochemical membrane-based sensors for rapidrelevant preliminary toxicity screens. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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