Reversed-Phase High-Performance Liquid Chromatography and Capillary Electrophoresis in the Stability Study of the Neuropeptide Growth Factor Antagonist [Arg6,D-Trp7,9,MePhe8]-Substance P {6-11}: A Comparative Study
| Autor: | W.J.M. Underberg, A. Bult, Jos H. Beijnen, Joop C. M. Waterval, J.L.E. Reubsaet, Jan Teeuwsen, E.H.M. Koster |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Electrophoresis
chemistry.chemical_classification Capillary electrochromatography Chromatography Molecular Structure Chemistry Capillary action Molecular Sequence Data Biophysics Antagonist Peptide Cell Biology Substance P Biochemistry High-performance liquid chromatography Peptide Fragments Kinetics Reaction rate constant Capillary electrophoresis Drug Stability Phase (matter) Amino Acid Sequence Nerve Growth Factors Molecular Biology Chromatography High Pressure Liquid |
| Zdroj: | Analytical Biochemistry. 220:98-102 |
| ISSN: | 0003-2697 |
| DOI: | 10.1006/abio.1994.1304 |
| Popis: | Reversed-phase high-performance liquid chromatography and capillary zone electrophoresis are widely used in protein and peptide analysis. Degradation of the basic peptide [Arg 6 , D-Trp 7,9 , MePhe 8 ]-substance P {6-11} (antagonist G) was monitored with reversed-phase high-performance liquid chromatography, free capillary zone electrophoresis, and capillary zone electrophoresis with a capillary cationic coating. Capillary zone electrophoresis with a dynamically coated capillary provided better separation between antagonist G and its degradation products (formed at pH/H — 13) than high-performance liquid chromatography and free zone capillary electrophoresis. Rate constants of the alkaline degradation of antagonist G measured with reversed-phase high-performance liquid chromatography and capillary zone electrophoresis with a dynamic coated capillary wall are similar whereas the values measured with free zone capillary electrophoresis are lower. Rate constants for the degradation of antagonist G in acidic media are comparable for the three techniques. It is concluded that capillary zone electrophoresis using a dynamic coating with Fluorad is the most suited of the above-mentioned techniques in analyzing antagonist G and its degradation products. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|