The influence of photodynamic therapy parameters on the inactivation of Candida spp: in vitro and in vivo studies
| Autor: | Lívia Nordi Dovigo, C. A. de Souza Costa, Ewerton Garcia de Oliveira Mima, Janaina Habib Jorge, Fiamma Buchinger Alves, V. S. Bagnato, Ana Cláudia Pavarina |
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| Rok vydání: | 2014 |
| Předmět: |
Candida glabrata
biology Chemistry medicine.medical_treatment Photodynamic therapy Condensed Matter Physics biology.organism_classification Industrial and Manufacturing Engineering Atomic and Molecular Physics and Optics In vitro Corpus albicans TERAPIA FOTODINÂMICA Microbiology Candida tropicalis In vivo Candida krusei medicine Candida albicans Instrumentation |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| Popis: | The influence of parameters of photodynamic therapy (PDT), such as pre-irradiation time (PIT), on the inactivation of Candida spp. was assessed in vitro and in vivo. Suspensions of Candida albicans, Candida tropicalis, Candida krusei and Candida glabrata were treated with Photogem®, incubated for 5, 10 or 15 min and illuminated with a blue LED light. Colonies were cultivated and log values of CFU ml−1 were analyzed by ANOVA and Kruskall–Wallis test. For in vivo evaluation, immunosuppressed mice were inoculated with C. albicans. PDT was performed on the dorsum of the tongue by topical administration of Photogem® and illumination after 5, 10 or 15 min. C. albicans was recovered from the tongue and the number of CFU ml−1 was analyzed by ANOVA and Tukey test. Animals were killed and the tongues were surgically removed for histological analysis. Susceptibility of Candida spp. suspensions to PDT was in decreasing order: C. albicans = C. tropicalis 0.05), both in vivo and in vitro. A significant reduction (p < 0.05) of log(CFU ml−1) of C. albicans from tongues of mice was observed with no adverse effects in the tissue. PDT was effective to inactivate in vitroCandida spp. and for reduction of C. albicans in vivo, independently of the PIT used. |
| Databáze: | OpenAIRE |
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