Dosage dependence of the effect of Ginkgo biloba on the rat retinal ganglion cell survival after optic nerve crush
| Autor: | L Xu, Mingliang Pu, H Zhan, K Ma, Jb Jonas, S Zhang |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Retinal Ganglion Cells
medicine.medical_specialty Cell Survival Nerve Crush medicine.medical_treatment Administration Oral Retinal ganglion Rats Sprague-Dawley chemistry.chemical_compound Ophthalmology medicine Animals Saline Retina Right optic nerve Dose-Response Relationship Drug Plant Extracts business.industry Ginkgo biloba Retinal Anatomy Rats Ganglion Disease Models Animal Neuroprotective Agents medicine.anatomical_structure chemistry Retinal ganglion cell Nerve Degeneration Optic nerve sense organs business Phytotherapy |
| Zdroj: | Eye. 23:1598-1604 |
| ISSN: | 1476-5454 0950-222X |
| Popis: | To investigate the dosage dependence of the Ginkgo biloba effect on retinal ganglion cell survival in the rat optic nerve crush model.The study included 56 Sprague-Dawley rats, the right optic nerve of which was crushed in a standardized manner. Two hours after the crush and once daily during the follow-up, the animals received intragastral applications of saline (saline group; n=13), or of a G. biloba extract of 0.25% concentration (n=14; low-dosage group), 1% concentration (n=15; medium-dosage group), or 4% concentration (n=14; high-dosage group). At 23 days after the optic nerve crush, the retinal ganglion cells were retrogradely labelled by injecting 3% fluorogold into the superior colliculi of the brain. At 4 weeks after baseline, the animals were killed. Retinal flat mount photographs were assessed for number and density of the retinal ganglion cells.The mean survival rate defined as the ratio of retinal ganglion cell density in the right eye with optic nerve crash divided by the retinal ganglion cell density in left eye without optic nerve intervention increased significantly (P0.001) from 58.4+/-9.0% in the saline group to 68.5+/-5.7% in the low-dosage group, to 73.7+/-6.4% in the medium-dosage group, and to 74.2+/-6.8% in the high-dosage group.Intragastral applications of a G. biloba extract applied after an experimental and standardized optic nerve crush in rats were associated with a higher survival rate of retinal ganglion cells in a dosage-dependent manner. |
| Databáze: | OpenAIRE |
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