H2Oh No! The importance of reporting your water source in your in vivo microbiome studies
| Autor: | Deanna L. Gibson, Jacqueline A. Barnett |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Research Report
0301 basic medicine Microbiology (medical) mice acidified water Water source Biology microbial ecology Microbiology Water Purification drinking water source 03 medical and health sciences 0302 clinical medicine Animals Microbiome bacteriome Clinical phenotype Environmental planning Gut microbiome Drinking Water Gastroenterology Jax vs Taconic Non-obese diabetic mice Reproducibility of Results Water Hydrogen-Ion Concentration 6. Clean water Gastrointestinal Microbiome in vivo experimental variable Muc2-/- mice spontaneous colitis model 030104 developmental biology Infectious Diseases Models Animal 030211 gastroenterology & hepatology Water treatment Water quality Commentary and Views |
| Zdroj: | Gut Microbes |
| ISSN: | 1949-0984 1949-0976 |
| DOI: | 10.1080/19490976.2018.1539599 |
| Popis: | Water is a fundamental part of any in vivo microbiome experiment however, it is also one of the most overlooked and underreported variables within the literature. Currently there is no established standard for drinking water quality set by the Canadian Council on Animal Care. Most water treatment methods focus on inhibiting bacterial growth within the water while prolonging the shelf-life of bottles once poured. When reviewing the literature, it is clear that some water treatment methods, such as water acidification, alter the gut microbiome of experimental animals resulting in dramatic differences in disease phenotype progression. Furthermore, The Jackson Lab, one of the world’s leading animal vendors, provides acidified water to their in-house animals and is often cited in the literature as having a dramatically different gut microbiome than animals acquired from either Charles River or Taconic. While we recognize that it is impossible to standardize water across all animal facilities currently conducting microbiome research, we hope that by drawing attention to the issue in this commentary, researchers will consider water source as an experimental variable and report their own water sources to facilitate experimental reproducibility. Moreover, researchers should be cognisant of potential phenotypic differences observed between commercial animal vendors due to changes in the gut microbiome as a result of various sources of water used. |
| Databáze: | OpenAIRE |
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