C3a-derived peptide binds to the type I FcepsilonR and inhibits proximal-coupling signal processes and cytokine secretion by mast cells
| Autor: | Hajna Péterfy, Anna Erdei, Gábor Tóth, Israel Pecht |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
MAPK/ERK pathway
MAP Kinase Signaling System Immunology Peptide binding Biology Cell Degranulation Cell Line Mice FYN LYN medicine Immunology and Allergy Animals Mast Cells Phosphorylation Protein kinase A Immunity Mucosal Receptors IgE Receptor Aggregation General Medicine Immunoglobulin E Mast cell Cell biology Rats Mice Inbred C57BL medicine.anatomical_structure src-Family Kinases Complement C3a Cytokines Cytokine secretion Signal transduction Mitogen-Activated Protein Kinases Peptides Protein Binding |
| Zdroj: | International immunology. 20(10) |
| ISSN: | 1460-2377 |
| Popis: | A peptide with the natural sequence derived from the complement component C3a, designated C3a7, and C3a9, having a modified sequence of that, was previously shown to inhibit the high-affinity IgER (FcepsilonRI)-induced secretory response of both mucosal and serosal-type mast cells. In addition, several processes that couple the FcepsilonRI stimulus to the cellular response were all suppressed in the presence of these peptides. Here, we show that peptide C3a9 binds to the FcepsilonRI on the surface of unperturbed mast cells (rat mucosal-type RBL-2H3 cell line) and remains bound even after FcepsilonRI-IgE aggregation by antigen as assessed by confocal microscopy. Moreover, that peptide interferes the initial steps of FcepsilonRI-coupling network. Namely, peptide binding to the FcepsilonRI beta-chain interrupts this chain's association with both src family protein tyrosine kinases Lyn and Fyn and enhances the internalization of the receptor. C3a9 was further found to inhibit the phosphorylation of two members of the mitogen-activated protein kinase family, extracellular signal-regulated kinase (ERK) and p38. Although ERK is usually activated via the ras-raf-mitogen-activated protein kinase/ERK kinase (MEK) pathway, our results show that C3a9 has no effect on the c-raf phosphorylation, suggesting that this complement-derived peptide inhibits ERK activation via an alternative route. C3a9 also inhibits the late-phase response to FcepsilonRI stimulus of bone marrow-derived mast cells, reducing secretion of the inflammatory cytokines IL-6 and tumor necrosis factor-alpha. Taken together, the consequence of its interference with the earliest steps of FcepsilonRI stimulus-response coupling and the C3a-derived peptide inhibits both the immediate and the late-phase responses of mast cells. |
| Databáze: | OpenAIRE |
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