| Autor: |
Yen-Chen Liao, James Fulcher, David Degnan, Sarah Williams, Lisa Bramer, Dušan Veličković, Kevin Zemaitis, Marija Veličković, Ryan Sontag, Ronald Moore, Ljiljana Paša-Tolić, Ying Zhu, Mowei Zhou |
| Rok vydání: |
2022 |
| Popis: |
Conventional proteomic approaches measure the averaged signal from mixed cell populations or bulk tissues, leading to the dilution of signals arising from subpopulations of cells that might serve as important biomarkers. Recent developments in bottom-up proteomics have enabled spatial mapping of cellular heterogeneity in tissue microenvironments. However, bottom-up proteomics cannot unambiguously define and quantify proteoforms, which are intact (i.e. functional) forms of proteins capturing genetic variations, alternatively spliced transcripts and post-translational modifications. Herein, we described a spatially resolved top-down proteomics (TDP) platform for proteoform identification and quantitation directly from tissue sections. The spatial TDP platform consisted of a nanoPOTS (nanodroplet Processing in One pot for Trace Samples)-based sample preparation system and an LCM (laser capture microdissection)-based cell isolation system. We improved the nanoPOTS sample preparation by adding benzonase in the extraction buffer to enhance the coverage of nucleus proteins. Using ~200 cultured cells as test samples, this approach increased total proteoform identifications from 493 to 700; with newly identified proteoforms primarily corresponding to nuclear proteins. To demonstrate the spatial TDP platform in tissue samples, we analyzed LCM-isolated tissue voxels from rat brain cortex and hypothalamus regions. We quantified 509 proteoforms within the union of TopPIC and TDPortal identifications to match with features from ProMex. Several proteoforms corresponding to the same gene exhibited mixed abundance profiles between two tissue regions, suggesting potential PTM-specific spatial distributions. The spatial TDP workflow has prospects for biomarker discovery at proteoform level from small tissue sections. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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