Analysis of LexA binding sites and transcriptomics in response to genotoxic stress inLeptospira interrogans
| Autor: | Janet L. Smith, Josefa B. da Silva, Renata M. A. da Costa, Juliana S Milanez, Renan H. Domingos, Alan D. Grossman, Helder I. Nakaya, Paulo Lee Ho, Luciane Schons-Fonseca |
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| Přispěvatelé: | Massachusetts Institute of Technology. Department of Biology, Schons-Fonseca, Luciane, Grossman, Alan D. |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Chromatin Immunoprecipitation DNA Repair Ultraviolet Rays DNA repair DNA damage 030106 microbiology Biology 03 medical and health sciences Bacterial Proteins Genetics SOS response SOS Response Genetics Gene Oligonucleotide Array Sequence Analysis Binding Sites Reverse Transcriptase Polymerase Chain Reaction Gene Expression Profiling Serine Endopeptidases EXPRESSÃO GÊNICA Genomics Gene Expression Regulation Bacterial Sequence Analysis DNA Chromosomes Bacterial Molecular biology 3. Good health ChIP-sequencing DNA binding site Repressor lexA Leptospira interrogans Energy Metabolism Cell Division DNA Damage Protein Binding Nucleotide excision repair |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP Oxford University Press Nucleic Acids Research |
| ISSN: | 1362-4962 0305-1048 |
| DOI: | 10.1093/nar/gkv1536 |
| Popis: | We determined the effects of DNA damage caused by ultraviolet radiation on gene expression in Leptospira interrogans using DNA microarrays. These data were integrated with DNA binding in vivo of LexA1, a regulator of the DNA damage response, assessed by chromatin immunoprecipitation and massively parallel DNA sequencing (ChIP-seq). In response to DNA damage, Leptospira induced expression of genes involved in DNA metabolism, in mobile genetic elements and defective prophages. The DNA repair genes involved in removal of photo-damage (e.g. nucleotide excision repair uvrABC, recombinases recBCD and resolvases ruvABC) were not induced. Genes involved in various metabolic pathways were down regulated, including genes involved in cell growth, RNA metabolism and the tricarboxylic acid cycle. From ChIP-seq data, we observed 24 LexA1 binding sites located throughout chromosome 1 and one binding site in chromosome 2. Expression of many, but not all, genes near those sites was increased following DNA damage. Binding sites were found as far as 550 bp upstream from the start codon, or 1 kb into the coding sequence. Our findings indicate that there is a shift in gene expression following DNA damage that represses genes involved in cell growth and virulence, and induces genes involved in mutagenesis and recombination. Conselho Nacional de Pesquisas (Brazil) Fundacao de Amparo a Pesquisa do Estado de Sao Paulo Fundacao Butantan National Institutes of Health (U.S.) (National Institute of General Medical Sciences (U.S.) Award GM41934) |
| Databáze: | OpenAIRE |
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