Automated ELISA On-Chip for the Detection of Anti-SARS-CoV-2 Antibodies
| Autor: | Liza P. Velarde-Calvillo, Carlos Aguilar-Avelar, Iram P. Rodriguez-Sanchez, Ricardo Garcia-Ramirez, Alejandro Abarca-Blanco, Miguel Esparza, Marisol Abarca-Blanco, Diana Aráiz-Hernández, Gladys Guadalupe Díaz-Armas, Brenda Soto-García, Juan F. Yee-de León, Jesús Rolando Delgado-Balderas, Everardo González-González, Elda A. Flores-Contreras, José R. Yee-de León |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
2019-20 coronavirus outbreak
Coronavirus disease 2019 (COVID-19) Computer science Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) microfluidics serology Enzyme-Linked Immunosorbent Assay TP1-1185 Computational biology spike protein Antibodies Viral Biochemistry Sensitivity and Specificity Analytical Chemistry Serology Limited access on-chip Medicine antibodies Humans Electrical and Electronic Engineering Instrumentation Pandemics biology business.industry SARS-CoV-2 Chemical technology Communication COVID-19 Serum samples Atomic and Molecular Physics and Optics Microplate Reader automated Immunology biology.protein ELISA Antibody business |
| Zdroj: | Sensors (Basel, Switzerland) Sensors, Vol 21, Iss 6785, p 6785 (2021) |
| ISSN: | 1424-8220 |
| Popis: | The COVID-19 pandemic has been the most critical public health issue in modern his-tory due to its highly infectious and deathly potential; and the limited access to massive, low-cost, and reliable testing has significantly worsened the crisis. The recovery and the vaccination of millions of people against COVID-19, have made serological tests highly relevant to identify the presence and levels of SARS-CoV-2 antibodies. Due to its advantages, microfluidic-based technologies represent an attractive alternative to the conventional testing methodologies used for these purposes. In this work, we describe the development of an automated ELISA on-chip capable of detecting anti-SARS-CoV-2 antibodies in serum samples from COVID-19 patients and vaccinated individuals. The colorimetric reactions were analyzed with a microplate reader. No statistically significant differences were observed when comparing the results of our automated ELISA on-chip against the ones obtained from a traditional ELISA on a microplate. Moreover, we demonstrated that it is possible to carry out the analysis of the colorimetric reaction by performing basic image analysis of photos taken with a smartphone, which constitutes a useful alternative when lacking specialized equipment or a laboratory setting. Our automated ELISA on-chip has the potential to be used in a clinical setting and mitigate some of the burden caused by testing deficiencies. |
| Databáze: | OpenAIRE |
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