Lactococcus lactis V7 inhibits the cell invasion of bovine mammary epithelial cells by Escherichia coli and Staphylococcus aureus
| Autor: | Jacques Robert Nicoli, Y. Le Loir, Aristóbolo M. Silva, B Seridan Assis, Sergine Even, Pierre Germon |
|---|---|
| Přispěvatelé: | Departemento de Microbiologia, Universidade Federal de Minas Gerais, UR Infectiologie animale et Santé publique (UR IASP), Institut National de la Recherche Agronomique (INRA), Departemento de Engenharia de Alimentos, Campus Sete Lagoas, Universidade Federal de São João del-Rei (UFSJ), Science et Technologie du Lait et de l'Oeuf (STLO), Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, ANR NABAB (ANR 08 ALIA 11) et INRA projet GISA Ruminflame), Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT) |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Chemokine
Antibiotics prévention des maladies lutte biologique medicine.disease_cause Bacterial Adhesion law.invention Probiotic law Mastitis Bovine 2. Zero hunger 0303 health sciences disease prevention inflammatory response cell invasion Endocytosis 3. Good health [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology Staphylococcus aureus invasion cellulaire cardiovascular system escherichia coli Microbiology (medical) staphylococcus aureus medicine.drug_class Enzyme-Linked Immunosorbent Assay bactérie probiotique Biology mastitis Microbiology lutte prophylactique Cell Line 03 medical and health sciences lactococcus lactis Antibiosis medicine Animals mammary probiotics Escherichia coli 030304 developmental biology 030306 microbiology Probiotics Interleukin-8 Lactococcus lactis Epithelial Cells biology.organism_classification medicine.disease Mastitis mammite Cell culture inflammation biology.protein Cattle [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition |
| Zdroj: | Beneficial Microbes Beneficial Microbes, Wageningen Academic Publishers 2015, 6 (6), pp.879-886. ⟨10.3920/BM2015.0019⟩ |
| ISSN: | 1876-2883 1876-2891 |
| DOI: | 10.3920/BM2015.0019⟩ |
| Popis: | Bovine mastitis, an inflammatory disease of the mammary gland often associated to bacterial infection, is the first cause of antibiotic use in dairy cattle. Because of the risk of antibioresistance emergence, alternative non-antibiotic strategies are needed to prevent or to cure bovine mastitis and reduce the antibiotic use in veterinary medicine. In this work, we investigated Lactococcus lactis V7, a strain isolated from the mammary gland, as a probiotic option against bovine mastitis. Using bovine mammary epithelial cell (bMEC) culture, and two representative strains for Escherichia coli and for Staphylococcus aureus, two major mastitis pathogens, we investigated L. lactis V7 ability to inhibit cell invasion (i.e. adhesion and internalization) of these pathogens into bMEC. L. lactis V7 ability to modulate the production of CXCL8, a key chemokine IL-8 responsible for neutrophil influx, in bMEC upon challenge with E. coli was investigated by an ELISA dosage of CXCL8 in bMEC culture supernatants. We showed that L. lactis V7 inhibited the internalisation of both E. coli and S. aureus strains into bMEC, whereas it inhibited the adhesion of only one out of the two S. aureus strains and of none of the E. coli strains tested. Investigation of the bMEC immune response showed that L. lactis V7 alone induced a slight increase in CXCL8 production in bMEC and that it increased the inflammatory response in bMEC challenged with the E. coli strains. Altogether these features of L. lactis V7 make it a potential promising candidate for a probiotic prevention strategy against bovine mastitis. |
| Databáze: | OpenAIRE |
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