Oncology drug discovery applications using the FMAT 8100 HTS system
| Autor: | Jennifer Y. Lee, Sheri Miraglia, Xiongwei Yan, Elana Swartzman, Susan Cornell-Kennon, Julia Mellentin-Michelotti, Charles Bruseo, Dennis S. France |
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| Rok vydání: | 2003 |
| Předmět: |
0301 basic medicine
High-throughput screening Antineoplastic Agents Apoptosis 01 natural sciences Biochemistry Analytical Chemistry 03 medical and health sciences Annexin Neoplasms Humans Annexin A5 Adaptor Proteins Signal Transducing GRB2 Adaptor Protein Pharmacology biology Kinase Proteins Molecular biology Enzyme assay 0104 chemical sciences 010404 medicinal & biomolecular chemistry 030104 developmental biology src-Family Kinases Homogeneous biology.protein Molecular Medicine Oncology drug Biological Assay Tyrosine kinase Biotechnology Proto-oncogene tyrosine-protein kinase Src |
| Zdroj: | Journal of biomolecular screening. 8(1) |
| ISSN: | 1087-0571 |
| Popis: | High-throughput screening (HTS) for potential anticancer agents requires a broad portfolio of assay platforms that may include kinase enzyme assays, protein-protein binding assays, and functional cell-based apoptosis assays. The authors have explored the use of fluorometric microvolume assay technology (the FMAT™ 8100 HTS System) in three distinct homogeneous HTS assays: (1) a Src tyrosine kinase enzyme assay, (2) a Grb2-SH2 protein-peptide interaction assay, and (3) an annexin V binding apoptosis assay. Data obtained from all three assays suggest that the FMAT system should facilitate the implementation of homogeneous assays for a wide variety of molecular targeted and cell-based screens. (Journal of Biomolecular Screening 2003:81-88) |
| Databáze: | OpenAIRE |
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