Beta-lapachone inhibits proliferation and induces apoptosis in retinoblastoma cell lines
| Autor: | Joan M. O'Brien, H R Shah, K.R. Van Quill, J. Qi, S.A. Howard, Vivian Weinberg, Michele C. Madigan, R.M. Conway |
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| Rok vydání: | 2007 |
| Předmět: |
Retinal Neoplasms
Caspase 3 Apoptosis Enzyme-Linked Immunosorbent Assay Caspase 7 medicine Tumor Cells Cultured Cytotoxic T cell Humans Cytotoxicity Cell Proliferation Dose-Response Relationship Drug Cell growth Retinoblastoma Chemistry medicine.disease Molecular biology eye diseases Ophthalmology Cell culture Enzyme Induction Immunology Reverse Transcriptase Inhibitors Naphthoquinones |
| Zdroj: | Eye (London, England). 22(3) |
| ISSN: | 0950-222X |
| Popis: | To investigate the cytotoxicity of beta-lapachone, a potent agent that may selectively target tumour cells, in retinoblastoma (RB) cell lines.Growth inhibitory effects of beta-lapachone were evaluated in Y79, WERI-RB1, and RBM human retinoblastoma cell lines. Pro-apoptotic effects of beta-lapachone were evaluated in Y79 cells by detection of caspase 3/7 activity, by enzyme-linked immunosorbent assay for nucleosome fragments, and by cellular morphological analysis.Beta-lapachone induced significant dose-dependent growth inhibitory effects in all three retinoblastoma cell lines. The 50% growth inhibitory concentration (IC(50)) of this agent was 1.9 microM in Y79 cells, 1.3 microM in WERI-RB1 cells, and 0.9 microM in RBM cells. Beta-lapachone also induced proapoptotic effects in RB cells. Treatment of Y79 cells with 1.9 microM beta-lapachone (IC(50)) resulted in a peak, fourfold induction of caspase 3/7 activity at 72 h post-treatment; a peak, 5.6-fold increase in nucleosome fragments at 96 h post-treatment; and a peak, 1.7-fold increase in the frequency of apoptotic cells at 48 h post-treatment, relative to vehicle-treated controls.Beta-lapachone induced potent cytotoxic effects in RB cell lines at low micromolar concentrations, suggesting this agent could be useful in the clinical management of RB. |
| Databáze: | OpenAIRE |
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