Transcriptional Complementarity in Breast Cancer: Application to Detection of Circulating Tumor Cells
| Autor: | Jiangchun Xu, Barbara K. Zehentner, Steven G. Reed, Cheryl Schmidt, Anthony Frudakis, Aristides Maltez Filho, Marcos Nolasco, John Jiang, Raymond L. Houghton, Xinqun Zhang, David H. Persing, David A. Molesh, Patrick C. Roche, Elizabeth A. Repasky, Davin C. Dillon, Roberto Badaró |
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| Rok vydání: | 2001 |
| Předmět: |
DNA
Complementary Transcription Genetic Down-Regulation Breast Neoplasms Polymerase Chain Reaction Magnetics Circulating tumor cell Mammaglobin Breast cancer Antigen Mammaglobin-A medicine Humans Uteroglobin Oligonucleotide Array Sequence Analysis biology Reverse Transcriptase Polymerase Chain Reaction Mammaglobin A Nucleic Acid Hybridization General Medicine Neoplastic Cells Circulating medicine.disease Molecular medicine Molecular biology Metastatic breast cancer Neoplasm Proteins Up-Regulation Real-time polymerase chain reaction biology.protein Cancer research Female |
| Zdroj: | Molecular Diagnosis. 6:79-91 |
| ISSN: | 1084-8592 |
| DOI: | 10.1007/bf03262038 |
| Popis: | Background: We used a combination of genetic subtraction, silicon DNA microarray analysis, and quantitative PCR to identify tissue- and tumor-specific genes as diagnostic targets for breast cancer. Methods and Results: From a large number of candidate antigens, several specific subsets of genes were identified that showed concordant and complementary expression profiles. Whereas transcriptional profiling of mammaglobin resulted in the detection of 70% of tumors in a panel of 46 primary and metastatic breast cancers, the inclusion of three additional markers resulted in detection of all 46 specimens. Immunomagnetic epithelial cell enrichment of circulating tumor cells from the peripheral blood of patients with metastatic breast cancer, coupled with RT-PCR-based amplification of breast tumor-specific transcripts, resulted in the detection of anchorage-independent tumor cells in the majority of patients with breast cancer with known metastatic disease. Conclusion: Complementation of mammaglobin with three additional genes in RT-PCR increases the detection of breast cancers in tissue and circulating tumor cells. |
| Databáze: | OpenAIRE |
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