Genetic Relatedness and Diversity of Staphylococcus aureus from Different Reservoirs: Humans and Animals of Livestock, Poultry, Zoo, and Aquaculture
| Autor: | Manuela Caniça, Vanessa Salgueiro, Vera Manageiro, Lurdes Clemente, Eugénia Ferreira, Narcisa M. Bandarra |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Microbiology (medical) human isolates 030106 microbiology MRSA Biology medicine.disease_cause Microbiology ST398 03 medical and health sciences Antibiotic resistance Virology Genotype medicine Animals Cefoxitin lcsh:QH301-705.5 Genetics Genetic diversity Phylogenetic tree Portugal Resistência aos Antimicrobianos SCCmec bacterial infections and mycoses Human Isolates animals 030104 developmental biology lcsh:Biology (General) Staphylococcus aureus Antibiotic Resistance Multilocus sequence typing medicine.drug |
| Zdroj: | Microorganisms Volume 8 Issue 9 Microorganisms, Vol 8, Iss 1345, p 1345 (2020) |
| Popis: | This article belongs to the Special Issue Deciphering the Crosswalk Dimension of Antibiotic Resistance between Different Reservoirs The main aim of this study was the characterization of antibiotic resistance mechanisms in 82 Staphylococcus aureus strains isolated from humans and animals. Antibiotic susceptibility testing was performed on all S. aureus isolates accordingly, and antibiotic-resistant genes were investigated by genotypic methods. The genetic diversity of S. aureus was studied through spa, multilocus sequence typing (MLST), and agr typing methods. The majority of S. aureus from human sources were resistant to cefoxitin (and harbor the mecA gene) and fluoroquinolones, whereas only four strains of S. aureus from animal sources revealed resistance to ciprofloxacin. In the set of S. aureus isolated from humans, the most frequent spa, MLST, and agr group were t032, ST22, and I, respectively. In strains from animal origin the most common spa, MLST, and agr group found were t2383, ST398, and III/not typable, respectively. S. aureus from humans and animals were identified either in clonal complexes CC5, CC30, and CC398, suggesting that they have the same putative founder in their evolution. Considering the three CCs encompassing strains from human and animal reservoirs with different spa-types, we can hypothesize that this might reflect an adaptation to different phylogenetic lineages in those reservoirs (host species) probably associated to genetic diversification of pre-existing strains. Vanessa Salgueiro had her Ph.D. fellowship granted by FCT (Fundação para a Ciência e a Tecnologia) with the reference SFRH/BD/133100/2017 co-financed by European Social Fund and the Operational Program for Human Capital (POCH), Portugal. We thank the following laboratories for sending human S. aureus isolates and laboratory records to the NRL-AMR-HAI: CHLC (M. Pinto), CHLO (E. Gonçalves), CHMA (H. Silva), CHMT (E. C. Cortes), CHON (A. Vicente), CHS (J. Duarte), CHSJ (J. Guimarães), CHTMAD (E. Costa), CHTS (M. E. Dias), CHUC (L. Albuquerque), CHVNG (A. Lira), HDFF (A. P. Vasco), HGO (J. Diogo), HH, Faial (J. Sachicumbi), H. Luz (P. Paixão), Hemobiolab (A. S. Jorge), HSMMB (A. Rocha); IPO Coimbra (F. Valido), LAC/A&D (M. Garcez), LAC/GAIA (A. Marques), and Laclibe (A. Gonçalves). info:eu-repo/semantics/publishedVersion |
| Databáze: | OpenAIRE |
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