Assessment of Markers of the Cell-Mediated Immune Response after Influenza Virus Infection in Frail Older Adults
| Autor: | Paul J. Drinka, Craig M. Upshaw, Peggy Krause, Stefan Gravenstein, Janet E. McElhaney, Jonathan W. Hooton |
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| Rok vydání: | 1998 |
| Předmět: |
Male
Microbiology (medical) Frail Elderly viruses Clinical Biochemistry Immunology Antibodies Viral Lymphocyte Activation medicine.disease_cause Article Granzymes Virus Disease Outbreaks Immune system Immunity Nasopharynx Influenza Human Influenza A virus medicine Humans Immunology and Allergy Aged Aged 80 and over Immunity Cellular biology Serine Endopeptidases Antibody titer virus diseases Middle Aged Virology Nursing Homes Granzyme B Influenza B virus Granzyme Influenza Vaccines biology.protein Cytokines Pharynx Female Antibody Biomarkers |
| Zdroj: | Clinical Diagnostic Laboratory Immunology. 5:840-844 |
| ISSN: | 1098-6588 1071-412X |
| Popis: | The purpose of this study was to determine whether measures of the cell-mediated immune response to influenza virus could be used as markers of influenza virus infection. We studied 23 subjects who developed upper respiratory, lower respiratory, or systemic symptoms during a small outbreak of influenza in a nursing home population. Influenza virus culture from nasopharyngeal swabs yielded influenza virus isolates from 7 of the 23 subjects. Only three of the subjects had a fourfold rise in antibody titer to the influenza virus antigen positivity after the infection. Granzyme B and cytokine levels were measured in peripheral blood mononuclear cells (PBMC) obtained from all subjects and stimulated with live influenza virus. Elevated granzyme B levels in virus-stimulated PBMC in combination with lower respiratory tract or systemic symptoms in study subjects was a significant predictor of culture-confirmed influenza virus infection compared to those from whom influenza virus could not be identified. Cytokine levels did not distinguish between the two groups in a similar type of analysis. Granzyme B in combination with the clinical profile of symptoms may be a useful retrospective marker for influenza virus infection. |
| Databáze: | OpenAIRE |
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