Vamana Couples Fat Signaling to the Hippo Pathway
| Autor: | Kenneth D. Irvine, Jyoti R. Misra |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Intracellular domain animal structures Myosins Protein Serine-Threonine Kinases Biology Article General Biochemistry Genetics and Molecular Biology src Homology Domains 03 medical and health sciences 0302 clinical medicine Planar cell polarity Myosin Animals Drosophila Proteins Wings Animal Molecular Biology Hippo signaling pathway Cell Membrane Intracellular Signaling Peptides and Proteins Cell Polarity Epistasis Genetic Cell Biology Physical interaction Cell biology Protein Transport Drosophila melanogaster Phenotype 030104 developmental biology Imaginal Discs Hippo signaling Mutation Signal transduction Cell Adhesion Molecules 030217 neurology & neurosurgery Protein Binding Signal Transduction Developmental Biology |
| Zdroj: | Developmental Cell. 39:254-266 |
| ISSN: | 1534-5807 |
| DOI: | 10.1016/j.devcel.2016.09.017 |
| Popis: | Summary The protocadherins Dachsous and Fat initiate a signaling pathway that controls growth and planar cell polarity by regulating the membrane localization of the atypical myosin Dachs. How Dachs is regulated by Fat signaling has remained unclear. Here we identify the vamana gene as playing a crucial role in regulating membrane localization of Dachs and in linking Fat and Dachsous to Dachs regulation. Vamana, an SH3-domain-containing protein, physically associates with and co-localizes with Dachs and promotes its membrane localization. Vamana also associates with the Dachsous intracellular domain and with a region of the Fat intracellular domain that is essential for controlling Hippo signaling and levels of Dachs. Epistasis experiments, structure-function analysis, and physical interaction experiments argue that Fat negatively regulates Dachs in a Vamana-dependent process. Our findings establish Vamana as a crucial component of the Dachsous-Fat pathway that transmits Fat signaling by regulating Dachs. |
| Databáze: | OpenAIRE |
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