Compression and Free Expansion of Single DNA Molecules in Nanochannels
| Autor: | Joshua D. Cross, John T. Mannion, Harold G. Craighead, Christian H. Reccius |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Electrophoresis
Models Molecular Materials science Compressive Strength General Physics and Astronomy Nanotechnology Physics::Fluid Dynamics Micromanipulation chemistry.chemical_compound Electric field Free expansion Molecule Computer Simulation chemistry.chemical_classification Physics::Biological Physics Quantitative Biology::Biomolecules DNA Polymer Microfluidic Analytical Techniques Quantitative Biology::Genomics Elasticity Transverse plane Models Chemical chemistry Chemical physics Nucleic Acid Conformation Stress Mechanical Elongation |
| Zdroj: | Physical Review Letters. 95 |
| ISSN: | 1079-7114 0031-9007 |
| DOI: | 10.1103/physrevlett.95.268101 |
| Popis: | We investigated compression and ensuing expansion of long DNA molecules confined in nanochannels. Transverse confinement of DNA molecules in the nanofluidic channels leads to elongation of their unconstrained equilibrium configuration. The extended molecules were compressed by electrophoretically driving them into porelike constrictions inside the nanochannels. When the electric field was turned off, the DNA strands expanded. This expansion, the dynamics of which has not previously been observable in artificial systems, is explained by a model that is a variation of de Gennes's polymer model. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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