In Vitro Studies Demonstrate Anticancer Activity of an Alkaloid of the Plant Gelsemium sempervirens
| Autor: | Sushil Kumar Mandal, Soumya Sundar Bhattacharyya, Naoual Boujedaini, Saili Paul, Raktim Biswas, Philippe Belon, Surajit Pathak, Anisur Rahman Khuda-Bukhsh |
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| Rok vydání: | 2008 |
| Předmět: |
0301 basic medicine
Serial dilution Cell Survival Chemical structure Tetrazolium Salts Antineoplastic Agents Apoptosis High-performance liquid chromatography General Biochemistry Genetics and Molecular Biology 03 medical and health sciences chemistry.chemical_compound Alkaloids 0302 clinical medicine Scopoletin Humans Potency Formazans Chromatography Dose-Response Relationship Drug Molecular Structure biology Plant Extracts G1 Phase Tincture biology.organism_classification Gelsemium Molecular Weight 030104 developmental biology chemistry 030220 oncology & carcinogenesis Two-dimensional nuclear magnetic resonance spectroscopy HeLa Cells |
| Zdroj: | Experimental Biology and Medicine. 233:1591-1601 |
| ISSN: | 1535-3699 1535-3702 |
| DOI: | 10.3181/0805-rm-181 |
| Popis: | The chemical structure of the main fluorescenting compound in the ethanolic extract (mother tincture) of the American yellow jasmine, Gelsemium sempervirens, was determined by employing 1H nuclear magnetic resonance (NMR), 13C NMR, mass spectroscopy, high-performance liquid chromatography (HPLC), correlation spectroscopy (COSY), and Fourier transform infrared (FTIR) spectroscopy analyses. Spectrofluorometric analysis has been made of the mother tincture and its agitated serial dilutions (up to 12th potency) prepared according to a homeopathic procedure in which serial, agitated dilutions were made separately in glass and polypropylene containers. The succussions were made by employing three different modes: hand jerk, sonication, and vortexing. The chemical formula of scopoletin, the main fluorescent compound, was determined to be C10H8O4 having a molecular weight of 192.17. Significant differences were noted between the remedies prepared in the two types of containers. Further, a comparison between any two methods of agitation revealed significant differences in fluorometric data of remedies at certain potency levels. The biological (anticancer) action of the crude extract, the alkaloid scopoletin, and 2C potency of Gelsemium sp were tested in vitro on the HeLa cell line through fluorescence microscopy, the 3(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay, and fluorescent activated cell sorting (FACS). The role of nanoparticles presumably derived from the containers, their orientation, and their interaction with the starting substance during the dynamization process initiated by different modes of agitation could possibly be attributed to the differences noted in the fluorometric data of potencies prepared in the two types of containers and among the three different means of succussion tested. |
| Databáze: | OpenAIRE |
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