| Autor: |
Gjert Knutsen, Torleiv Lien, Olav Valle |
| Rok vydání: |
1981 |
| Předmět: |
|
| Zdroj: |
Journal of Biochemical and Biophysical Methods. 4:271-277 |
| ISSN: |
0165-022X |
| DOI: |
10.1016/0165-022x(81)90067-1 |
| Popis: |
By using the fluorescence enhancement of ethidium bromide bound to nucleic acid, a very rapid, simple and sensitive assay of DNA in the green alga Chlamydomonas has been devised. Total fluorescence (DNA + RNA) was determined by complex formation with ethidium bromide in a cell lysate made by mixing cell samples with lauroyl sarcosinate, EDTA and NaOH and incubating the mixture for 5 min at room temperature followed by neutralization. For determination of DNA the RNA was digested by incubating the cell sample in the alkaline lysis solution for 45 min at 60°C followed by neutralization, and complex formation with ethidium bromide. Quenching of the fluorescence due to cellular pigments was corrected for by usiong an internal DNA standard. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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