Distribution pattern of tumor infiltrating lymphocytes and tumor microenvironment composition as prognostic indicators in anorectal malignant melanoma

Autor: Seung-Mo Hong, Jin Cheon Kim, Mi Ju Kim, Bilal Mustafa, Young Il Kim, Gowun Jeong, Sung-Min Ahn, So-Woon Kim, Seok-Byung Lim, Chang Sik Yu, In Ja Park
Rok vydání: 2021
Předmět:
Male
0301 basic medicine
Oncology
medicine.medical_specialty
Pathology
CD3 Complex
Databases
Factual
medicine.medical_treatment
Antigens
Differentiation
Myelomonocytic
CD8-Positive T-Lymphocytes
B7-H1 Antigen
Pathology and Forensic Medicine
03 medical and health sciences
Lymphocytes
Tumor-Infiltrating
0302 clinical medicine
Antigens
CD
Internal medicine
Tumor-Associated Macrophages
Biomarkers
Tumor
Tumor Microenvironment
medicine
Humans
Melanoma
Aged
Retrospective Studies
Extracellular Matrix Proteins
Tumor microenvironment
CD68
Tumor-infiltrating lymphocytes
business.industry
FOXP3
Forkhead Transcription Factors
Immunotherapy
Middle Aged
Anus Neoplasms
Prognosis
medicine.disease
Hyaluronan Receptors
Phenotype
030104 developmental biology
030220 oncology & carcinogenesis
Immunohistochemistry
Female
Neoplasm Recurrence
Local
business
CD163
Zdroj: Modern Pathology. 34:141-160
ISSN: 0893-3952
Popis: Anorectal malignant melanoma (ARMM) is a rare disease with poor prognosis. Determining ARMM prognosis precisely is difficult due to the lack of proper assessment techniques. Immunotherapy has proven effective against cutaneous malignant melanoma and may show efficacy in ARMM. Herein, we assessed the immune profile of ARMM to identify possible prognostic biomarkers. Twenty-two ARMM formalin-fixed and paraffin-embedded samples were evaluated using an nCounter® PanCancer Immune Profiling Panel. Validation was performed through immunohistochemical staining for CD3, CD8, Foxp3, CD68, CD163, and PD-L1. RNA analysis revealed significantly decreased scores for pathways involved in cell regulation and function, as well as chemokines, in recurrent patients compared to nonrecurrent patients. In cell-type profiling, the recurrent cases displayed significantly low tumor infiltrating lymphocyte (TIL) scores. Recurrence/death prediction models were defined using logistic regression and showed significantly lower scores in recurrent and deceased patients (all, P
Databáze: OpenAIRE
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