Distribution pattern of tumor infiltrating lymphocytes and tumor microenvironment composition as prognostic indicators in anorectal malignant melanoma
Autor: | Seung-Mo Hong, Jin Cheon Kim, Mi Ju Kim, Bilal Mustafa, Young Il Kim, Gowun Jeong, Sung-Min Ahn, So-Woon Kim, Seok-Byung Lim, Chang Sik Yu, In Ja Park |
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Rok vydání: | 2021 |
Předmět: |
Male
0301 basic medicine Oncology medicine.medical_specialty Pathology CD3 Complex Databases Factual medicine.medical_treatment Antigens Differentiation Myelomonocytic CD8-Positive T-Lymphocytes B7-H1 Antigen Pathology and Forensic Medicine 03 medical and health sciences Lymphocytes Tumor-Infiltrating 0302 clinical medicine Antigens CD Internal medicine Tumor-Associated Macrophages Biomarkers Tumor Tumor Microenvironment medicine Humans Melanoma Aged Retrospective Studies Extracellular Matrix Proteins Tumor microenvironment CD68 Tumor-infiltrating lymphocytes business.industry FOXP3 Forkhead Transcription Factors Immunotherapy Middle Aged Anus Neoplasms Prognosis medicine.disease Hyaluronan Receptors Phenotype 030104 developmental biology 030220 oncology & carcinogenesis Immunohistochemistry Female Neoplasm Recurrence Local business CD163 |
Zdroj: | Modern Pathology. 34:141-160 |
ISSN: | 0893-3952 |
Popis: | Anorectal malignant melanoma (ARMM) is a rare disease with poor prognosis. Determining ARMM prognosis precisely is difficult due to the lack of proper assessment techniques. Immunotherapy has proven effective against cutaneous malignant melanoma and may show efficacy in ARMM. Herein, we assessed the immune profile of ARMM to identify possible prognostic biomarkers. Twenty-two ARMM formalin-fixed and paraffin-embedded samples were evaluated using an nCounter® PanCancer Immune Profiling Panel. Validation was performed through immunohistochemical staining for CD3, CD8, Foxp3, CD68, CD163, and PD-L1. RNA analysis revealed significantly decreased scores for pathways involved in cell regulation and function, as well as chemokines, in recurrent patients compared to nonrecurrent patients. In cell-type profiling, the recurrent cases displayed significantly low tumor infiltrating lymphocyte (TIL) scores. Recurrence/death prediction models were defined using logistic regression and showed significantly lower scores in recurrent and deceased patients (all, P |
Databáze: | OpenAIRE |
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