Genetic variation of wild Puumala viruses within the serotype, local rodent populations and individual animal
| Autor: | Heikki Lehväslaiho, Tatiana Mikhailova, Irina N. Gavrilovskaya, Heikki Henttonen, Olli Vapalahti, Natalia Apekina, Jukka Niemimaa, Antti Vaeri, Juha Laakkonen, M. Brummer-Korvenkontio, Alexander Plyusnin |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Orthohantavirus
Cancer Research Genes Viral Molecular Sequence Data Rodentia Viral quasispecies Biology law.invention 03 medical and health sciences Capsid Protein sequencing law Virology Genetic variation Animals Amino Acid Sequence Serotyping Gene Phylogeny Polymerase chain reaction 030304 developmental biology Hantavirus Genetics 0303 health sciences Base Sequence Phylogenetic tree 030306 microbiology Viral Core Proteins Genetic Variation biology.organism_classification 3. Good health Infectious Diseases DNA Viral Puumala virus |
| Zdroj: | University of Helsinki |
| ISSN: | 0168-1702 |
| DOI: | 10.1016/0168-1702(95)00038-r |
| Popis: | Reverse transcriptase polymerase chain reaction cloning and sequencing were used to determine the range of S gene/N protein variability in wild Puumala virus (PUU) strains and to study phylogenetic relationships between two groups of strains which originated from Finland and from European Russia. Analyses of the nucleotide and predicted aino acid sequences showed: (1) all PUU strains shared a common ancient ancestor; and (2) the more recent ancestors were different for the Finnish branch and the Russian branch of PUU strains. A cluster of amino acid substitutions in the N protein of Finnish strains was found; this cluster was located within a highly variable region of the molecule carrying B-cell epitopes (Vapalahti et al., J. Med. Virol., 1995, in press). Different levels of S gene/N protein diversity of PUU were revealed supporting the view of geographical clustering of genetic variants. Puumala virus from individual voles was found to be a complex mixture of closely related variant s-quasispecies. The ratio of non-silent to silent nucleotide mutations registered in the S genes/N proteins of PUU quasispecies was 4- to 16-fold higher than that in Puumala virus strains, resulting in a more wide range of quasispecies N protein sequence diversity. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect