Spatio-temporal expression of Drosophila mitochondrial transcription factor A during development
| Autor: | Ippei Sakimoto, Fumiko Hirose, Shizuka Murakami, Kei Ichi Takata, Yoshihiro H. Inoue, Kaori Shimanouchi, Kengo Sakaguchi |
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| Rok vydání: | 2003 |
| Předmět: |
Male
Regulation of gene expression Time Factors Base Sequence Gene Expression Regulation Developmental Nuclear Proteins Promoter Cell Biology General Medicine TCF4 Biology TFAM Molecular biology DNA-Binding Proteins Mitochondrial Proteins Drosophila melanogaster Sp3 transcription factor Transcription (biology) Mutation Animals Drosophila Proteins Spermatogenesis Transcription factor Drosophila Protein Transcription Factors |
| Zdroj: | Cell Biology International. 27:361-374 |
| ISSN: | 1065-6995 |
| Popis: | We previously reported cloning of the Drosophila mitochondrial transcription factor A (D-mtTFA) gene, and characterization of a recombinant protein. In this report, the expression and distribution patterns of D-mtTFA during development are described. D-mtTFA mRNA and its protein product were found to be abundant in cells of tissues undergoing both proliferation and polytenization. Furthermore, a DRE-like sequence present in the D-mtTFA gene showed promotor activity in Drosophila Kc cells. In addition, D-mtTFA was detected in brain throughout the developmental process, as well as in non-proliferating tissues, such as flight muscle and cardia, and was also found in spermatids of imagos. D-mtTFA possesses a nuclear-targeting sequence, and is present in the nucleus at the syncytial stage, where bundles of 64 spermatids are present during spermatogenesis. The results suggest that D-mtTFA not only contributes to the mitochondrial DNA transcription and replication system, but also has a role in cell proliferation and development, including spermatogenesis. |
| Databáze: | OpenAIRE |
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