The development of IgE+ memory B cells following primary IgE immune responses
| Autor: | M H Kosco-Vilbois, Fred D. Finkelman, C Heusser, G Le Gros, K Einsle, N Schultze, S Walti |
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| Rok vydání: | 1996 |
| Předmět: |
T cell
Immunology Antibodies Helminth chemical and pharmacologic phenomena Immunoglobulin E Mice Immune system Antibody Specificity medicine Immunology and Allergy Animals Nippostrongylus brasiliensis Memory B cell B-Lymphocytes Mice Inbred BALB C biology CD23 biology.organism_classification medicine.anatomical_structure Immunization biology.protein Nippostrongylus Antibody Immunologic Memory |
| Zdroj: | European journal of immunology. 26(12) |
| ISSN: | 0014-2980 |
| Popis: | We studied whether long-lived IgE+ memory B cells develop following three types of primary IgE immune responses. Immunization of mice with anti-IgD antibody induced a T cell-dependent, interleukin (IL)-4-dependent primary IgE response and the formation of IgE isotype switched (IgE+) memory B cells. These IgE+ memory B cells could be stimulated in vivo by injection with goat anti-IgE antibodies to produce a profound IL-4-independent memory IgE response. By contrast, both infection of mice with Nippostrongylus brasiliensis or repeated immunization with benzylpenicilloyl-keyhole limpet hemocyanin (BPO-KLH) in alum stimulated good primary IgE responses and profound memory T cell-dependent antigen-specific IgE responses, but failed to induce the development of long lived IgE+ memory B cells because they could not be recalled with goat anti-IgE antibodies. Mice receiving double immunizations combining anti-IgD with either N. brasiliensis infection or BPO-KLH immunization mounted significant goat anti-IgE-induced secondary IgE responses, but no N. brasiliensis or BPO-KLH-specific IgE could be detected. This indicates that the N. brasiliensis and BPO-KLH induced immune responses do not suppress the development of IgE+ B cells, but rather, do not provide the necessary conditions for their formation. Taken together these data indicate that long-lived IgE+ B cells fail to develop during the primary IgE response to N. brasiliensis infection or BPO-KLH immunization. By contrast, significant numbers of IgE+ memory B cells form during the primary IgE immune response induced by anti-IgD immunization. Our observations suggest that immunization protocols involving membrane IgD cross-linking and limited duration of cognate T cell help are necessary for the formation of IgE+ memory B cells. It will be important to determine the relevance of membrane IgD interaction with allergens, as this would influence the design of new therapies for the treatment of allergy and asthma. |
| Databáze: | OpenAIRE |
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