A curcumin-analogous fluorescent sensor for cysteine detection with a bilateral-response click-like mechanism
| Autor: | Yu-Shun Yang, Xiang-Jun Fan, Sheng-Jin Liu, Zhen Li, Fang Fang |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Curcumin
High selectivity Covalent binding chemical and pharmacologic phenomena 02 engineering and technology 010402 general chemistry 01 natural sciences Analytical Chemistry chemistry.chemical_compound Molecule Humans Cysteine Instrumentation Homocysteine Spectroscopy Fluorescent Dyes Chemistry Glutathione 021001 nanoscience & nanotechnology Fluorescence Atomic and Molecular Physics and Optics 0104 chemical sciences Biophysics Azide 0210 nano-technology HeLa Cells |
| Zdroj: | Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy. 245 |
| ISSN: | 1873-3557 |
| Popis: | A novel curcumin-analogous fluorescent sensor, DNP, was developed for cysteine detection with a bilateral-response click-like mechanism. DNP indicated high selectivity and practical sensitivity. It could recognize Cys from other biologically relevant molecules, especially, from GSH and Hcy. The most interesting point was that, with typical azide groups for sensing, DNP indicated a covalent binding procedure with Cys instead of a presupposed simple reduction for reductive sulfide. Moreover, the recognition occurred at both sides of the sensor. DNP could be utilized into the detection of endogenous and exogenous Cys in living cells. Though the specific optical performances of DNP still need optimization, this work supplied novel information for broadening the vision on fluorophores and mechanisms, for the monitoring of Cys and even other sulfur-containing species. |
| Databáze: | OpenAIRE |
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