Generation of one iPSC line (IMEDEAi007-A) by Sendai Virus transduction of PBMCs from a Psoriasis donor
| Autor: | Mónica Castresana, Aarne Fleischer, Carmelo Gómez-Martínez, Sara Vallejo-Diez, Daniel Bachiller, José María Martín-Fernández, Almudena Sánchez-Gilabert |
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| Přispěvatelé: | Ministerio de Economía y Competitividad (España), Eusko Jaurlaritza, Ministerio de Ciencia e Innovación (España) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Transgene Cell Biology General Medicine Biology biology.organism_classification medicine.disease Peripheral blood mononuclear cell Sendai virus 03 medical and health sciences Transduction (genetics) 030104 developmental biology 0302 clinical medicine SOX2 lcsh:Biology (General) Psoriasis Immunology Genetic predisposition medicine Reprogramming lcsh:QH301-705.5 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Stem Cell Research, Vol 47, Iss, Pp 101917-(2020) Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 1873-5061 |
| Popis: | Psoriasis is a chronic inflammatory skin disease that speeds up the life cycle of skin cells, forming scales and red patches that are itchy and sometimes painful. It is a complex disease of autoimmune origin and genetic predisposition with more than 10 different loci associated. Here we described the production of an iPSC line generated by Sendai Virus (Klf4, Oct3/4, Sox2 and c-Myc) reprogramming of Peripheral Blood Mononuclear Cells (PBMCs) from a Psoriasis patient. The iPSC line generated has normal 46XY karyotype, is free of SeV genome and transgenes insertions, express high levels of pluripotency markers and can differentiate into all three germ layers. Funding was provided by the Spanish Ministry for Science and Innovation (RTC-2016-5324-1). JMMF was a postdoctoral Berrikertu fellow from the Basque government. AF was a recipient of Juan de la Cierva (JCI-2006-2675) and Torres Quevedo (PTQ-16-08496) postdoctoral fellowships from the Spanish Ministry for Science and Innovation. |
| Databáze: | OpenAIRE |
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