Proteomic and functional analyses of Nelumbo nucifera annexins involved in seed thermotolerance and germination vigor
| Autor: | Keqiang Wu, Shangzhi Huang, Huhui Chen, Yu Ding, Yuliang Zhou, Yin Li, Pu Chu, Liwen Jiang, Edward W. T. Tsang |
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| Rok vydání: | 2011 |
| Předmět: |
Proteomics
DNA Complementary Time Factors Annexins Transgene Molecular Sequence Data Lotus Arabidopsis Germination Annexin Plant Science Nelumbo Mass Spectrometry Lipid peroxidation chemistry.chemical_compound Gene Expression Regulation Plant Stress Physiological Botany Escherichia coli Genetics Electrophoresis Gel Two-Dimensional Amino Acid Sequence Plant Proteins biology Gene Expression Profiling Temperature food and beverages Plants Genetically Modified biology.organism_classification Adaptation Physiological Seed vigor Recombinant Proteins Sacred lotus Cell biology Protein Transport chemistry Seed thermotolerance Seeds biology.protein Ectopic expression Subcellular Fractions Peroxidase |
| Zdroj: | Planta. 235:1271-1288 |
| ISSN: | 1432-2048 0032-0935 |
| DOI: | 10.1007/s00425-011-1573-y |
| Popis: | Annexins are multifunctional proteins characterized by their capacity to bind calcium ions and negatively charged lipids. Although there is increasing evidence implicating their importance in plant stress responses, their functions in seeds remain to be further studied. In this study, we identified a heat-induced annexin, NnANN1, from the embryonic axes of sacred lotus (Nelumbo nucifera Gaertn.) using comparative proteomics approach. Moreover, the expression of NnANN1 increased considerably in response to high-temperature treatment. Quantitative real-time PCR (qRT-PCR) revealed that the transcripts of NnANN1 were detected predominantly during seed development and germination in sacred lotus, implicating a role for NnANN1 in plant seeds. Ectopic expression of NnANN1 in Arabidopsis resulted in enhanced tolerance to heat stress in transgenic seeds. In addition, compared to the wild-type seeds, transgenic seeds ectopically expressing NnANN1 exhibited improved resistance to accelerated aging treatment used for assessing seed vigor. Furthermore, transgenic seeds showed enhanced peroxidase activities, accompanied with reduced lipid peroxidation and reduced ROS release levels compared to the wild-type seeds. Taken together, these results indicate that NnANN1 plays an important role in seed thermotolerance and germination vigor. |
| Databáze: | OpenAIRE |
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