Threonyl-tRNA synthetase overexpression correlates with angiogenic markers and progression of human ovarian cancer

Autor: Karen M. Lounsbury, Cheung Wong, Midori Eckenstein, Christopher S. Francklyn, Takamaru Ashikaga, Sharon L. Mount, Mercedes Rincon, Theresa L. Wellman
Rok vydání: 2014
Předmět:
Vascular Endothelial Growth Factor A
Multivariate Cox analysis
Cancer Research
tRNA synthetase
Angiogenesis
viruses
Carcinoma
Ovarian Epithelial
Biology
complex mixtures
03 medical and health sciences
0302 clinical medicine
Immune system
Surgical oncology
Cell Line
Tumor
otorhinolaryngologic diseases
Biomarkers
Tumor
Threonine-tRNA Ligase
Genetics
medicine
Protein biosynthesis
Extracellular
Humans
Neoplasms
Glandular and Epithelial
030304 developmental biology
Ovarian Neoplasms
0303 health sciences
Tumor microenvironment
Neovascularization
Pathologic
Tumor Necrosis Factor-alpha
Serous papillary ovarian cancer
medicine.disease
Database analysis
Survival Analysis
3. Good health
Oncology
030220 oncology & carcinogenesis
Immunology
Cancer research
Female
Stem cell
Ovarian cancer
Research Article
Zdroj: BMC Cancer
ISSN: 1471-2407
DOI: 10.1186/1471-2407-14-620
Popis: Background Ovarian tumors create a dynamic microenvironment that promotes angiogenesis and reduces immune responses. Our research has revealed that threonyl-tRNA synthetase (TARS) has an extracellular angiogenic activity separate from its function in protein synthesis. The objective of this study was to test the hypothesis that TARS expression in clinical samples correlates with angiogenic markers and ovarian cancer progression. Methods Protein and mRNA databases were explored to correlate TARS expression with ovarian cancer. Serial sections of paraffin embedded ovarian tissues from 70 patients diagnosed with epithelial ovarian cancer and 12 control patients were assessed for expression of TARS, vascular endothelial growth factor (VEGF) and PECAM using immunohistochemistry. TARS secretion from SK-OV-3 human ovarian cancer cells was measured. Serum samples from 31 tissue-matched patients were analyzed by ELISA for TARS, CA-125, and tumor necrosis factor-α (TNF-α). Results There was a strong association between the tumor expression of TARS and advancing stage of epithelial ovarian cancer (p
Databáze: OpenAIRE
Externí odkaz: