| Autor: |
Jun-Xiong Huang, Georges Guiochon |
| Rok vydání: |
1989 |
| Předmět: |
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| Zdroj: |
Journal of Chromatography B: Biomedical Sciences and Applications. 492:431-467 |
| ISSN: |
0378-4347 |
| DOI: |
10.1016/s0378-4347(00)84477-1 |
| Popis: |
The basic differences between the problems encountered in the development of the analytical and preparative applications of high-performance liquid chromatography are analyzed and the principles of optimization of a preparative separation outlined. The application of these principles to the purification of peptides and proteins are discussed with emphasis on the specific problems raised by the separation of high-molecular-mass compounds. The relative advantages and inconveniences of displacement and overloaded elution are presented. The applications of the various retention modes of liquid chromatography (ion exchange, reversed-phase adsorption, hydrophobic interactions, size exclusion and bioaffinity) to the separation of various peptide and protein mixtures and/or to the extraction and purification of some of these compounds are reviewed. Separation performance reported in the literature are summarized in table form. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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