Evidence for incorporation of thymidine into deoxyribonucleic acid in airborne bacterial cells
| Autor: | P. Straat, Mark A Chatigny, R. L. Dimmick, H. Wolochow |
|---|---|
| Rok vydání: | 1977 |
| Předmět: |
DNA
Bacterial Air Microbiology Applied Microbiology and Biotechnology chemistry.chemical_compound Formaldehyde Serratia marcescens High humidity Aerosols Growth medium Ecology biology Radiochemistry biology.organism_classification In vitro Aerosol chemistry Biochemistry Thymidine Cell Division Bacteria DNA Research Article Food Science Biotechnology |
| Zdroj: | Applied and Environmental Microbiology. 34:292-296 |
| ISSN: | 1098-5336 0099-2240 |
| DOI: | 10.1128/aem.34.3.292-296.1977 |
| Popis: | As part of an effort to discover whether bacteria might propagate within airborne particles, we studied the incorporation of thymidine into the trichloroacetic acid-insoluble fraction of airborne cells of Serratia marcescens to seek evidence of the possible formation of new DNA. Two aerosols, one of S. marcescens and another of [3H]thymidine ([3H]dT) suspended in growth medium were caused to aggregate in air just prior to directing the aerosols into rotating-drum aerosol storage chambers. The age of the S. marcescens culture and other conditions for maximizing ([3H]dT) uptake were selected on the basis of prior in vitro trials. With 10-h cultures and addition of 2-deoxyadenosine to the [3H]dT, we showed that [3H]dT is incorporated into the trichloroacetic acid-insoluble fraction of cells recovered 6 h after aerosols were stored under the conditions of high humidity and 30 degrees C. Tests conducted in the same manner with Formalin-killed S. marcescens ruled out the possibility of adsorptive carry-over of [3H]dT. As much as 20 times more activity was found in the trichloroacetic acid-insoluble fraction of live cells than of dead cells. |
| Databáze: | OpenAIRE |
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