Lysosome triggered near-infrared fluorescence imaging of cellular trafficking processes in real time
| Autor: | Angela Panarella, Jeremy C. Simpson, Dimitri Scholz, Shane Cheung, Donal F. O'Shea, William M. Gallagher, Emer Conroy, Marina Morgunova, Marco Grossi, Marta Terrile |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Near-Infrared Fluorescence Imaging
Fluorophore Science Chemical biology General Physics and Astronomy Breast Neoplasms Cell Communication 02 engineering and technology Adenocarcinoma 010402 general chemistry Endocytosis 01 natural sciences Article General Biochemistry Genetics and Molecular Biology Green fluorescent protein Mice chemistry.chemical_compound In vivo Cell Line Tumor Animals Humans Fluorescent Dyes Spectroscopy Near-Infrared Multidisciplinary Molecular Structure Chemistry Optical Imaging Neoplasms Experimental General Chemistry 021001 nanoscience & nanotechnology Fluorescence 0104 chemical sciences Cell biology Microscopy Fluorescence Female Lysosomes 0210 nano-technology Preclinical imaging |
| Zdroj: | Nature Communications, Vol 7, Iss 1, Pp 1-13 (2016) Nature Communications |
| ISSN: | 2041-1723 |
| DOI: | 10.1038/ncomms10855 |
| Popis: | Bioresponsive NIR-fluorophores offer the possibility for continual visualization of dynamic cellular processes with added potential for direct translation to in vivo imaging. Here we show the design, synthesis and lysosome-responsive emission properties of a new NIR fluorophore. The NIR fluorescent probe design differs from typical amine functionalized lysosomotropic stains with off/on fluorescence switching controlled by a reversible phenol/phenolate interconversion. Emission from the probe is shown to be highly selective for the lysosomes in co-imaging experiments using a HeLa cell line expressing the lysosomal-associated membrane protein 1 fused to green fluorescent protein. The responsive probe is capable of real-time continuous imaging of fundamental cellular processes such as endocytosis, lysosomal trafficking and efflux in 3D and 4D. The advantage of the NIR emission allows for direct translation to in vivo tumour imaging, which is successfully demonstrated using an MDA-MB-231 subcutaneous tumour model. This bioresponsive NIR fluorophore offers significant potential for use in live cellular and in vivo imaging, for which currently there is a deficit of suitable molecular fluorescent tools. Real time cellular fluorescence imaging requires a probe that displays high degrees of localisation, low toxicity and good photostability. Here, the authors report a near infrared fluorophore that displays pH-sensitive fluorescence based on phenol/phenolate interconversion, showing real time imaging of cellular processes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|