Absence of XMRV retrovirus and other murine leukemia virus-related viruses in patients with chronic fatigue syndrome
| Autor: | Christopher J. Leonard, Ronald W. Hughen, Robert Schlaberg, Ashley M. Bunker, Lucinda Bateman, Kathleen C. Light, Clifford Shin, Ila R. Singh, Alan R. Light |
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| Rok vydání: | 2011 |
| Předmět: |
musculoskeletal diseases
Adult viruses Xenotropic murine leukemia virus-related virus Immunology Blotting Western Enzyme-Linked Immunosorbent Assay Virus Replication Microbiology Polymerase Chain Reaction Virus Retrovirus Virology Murine leukemia virus medicine Chronic fatigue syndrome Humans Gammaretrovirus DNA Primers Fatigue Syndrome Chronic biology Base Sequence virus diseases biology.organism_classification medicine.disease Leukemia Viral replication Insect Science Pathogenesis and Immunity Female |
| Zdroj: | Journal of virology. 85(14) |
| ISSN: | 1098-5514 |
| Popis: | Chronic fatigue syndrome (CFS) is a multisystem disorder characterized by prolonged and severe fatigue that is not relieved by rest. Attempts to treat CFS have been largely ineffective primarily because the etiology of the disorder is unknown. Recently, CFS has been associated with xenotropic murine leukemia virus-related virus (XMRV) as well as other murine leukemia virus (MLV)-related viruses, though not all studies have found these associations. We collected blood samples from 100 CFS patients and 200 self-reported healthy volunteers from the same geographical area. We analyzed these in a blind manner using molecular, serological, and viral replication assays. We also analyzed samples from patients in the original study that reported XMRV in CFS patients. We did not find XMRV or related MLVs either as viral sequences or infectious viruses, nor did we find antibodies to these viruses in any of the patient samples, including those from the original study. We show that at least some of the discrepancy with previous studies is due to the presence of trace amounts of mouse DNA in the Taq polymerase enzymes used in these previous studies. Our findings do not support an association between CFS and MLV-related viruses, including XMRV, and the off-label use of antiretrovirals for the treatment of CFS does not seem justified at present. |
| Databáze: | OpenAIRE |
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