Impaired bacterial clearance and trapping in obstructive jaundice
| Autor: | Kirby R. Gross, Thomas R. Weber, David A. Plager, Jay L. Grosfeld, Raoul S. Rosenthal, Meredith T. Hull, Donald Ross, Schmuel Katz |
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| Rok vydání: | 1984 |
| Předmět: |
Male
medicine.medical_specialty Pathology medicine.medical_treatment Splenectomy Spleen Phagocytic dysfunction Kidney Gastroenterology Sepsis Phagocytosis Internal medicine medicine Escherichia coli Animals Lung Mononuclear Phagocyte System Escherichia coli Infections Common Bile Duct Cholestasis Common bile duct business.industry Hepatobiliary disease Body Weight Rats Inbred Strains Organ Size Jaundice medicine.disease Rats medicine.anatomical_structure Blood Liver Surgery medicine.symptom business Research Article |
| Zdroj: | Annals of surgery. 199(1) |
| ISSN: | 0003-4932 |
| Popis: | Sepsis is a major cause of mortality in patients with common bile duct obstruction. To define possible contributing factors to this phenomenon, this study evaluates the effect of biliary obstruction on the intravascular clearance and organ trapping of viable Escherichia coli using a rat model. Adult male Sprague-Dawley rats were placed in three groups: Group I controls had sham operation, Group II had division and ligation of common bile duct (CDL), and Group III underwent splenectomy. At 21 days following operation 10(9) radiolabeled E. coli were injected intravenously. At varying intervals after infusion, blood samples were obtained for clearance study. At 10 minutes, bacterial distribution in the liver, spleen, kidneys, and lungs was determined (expressed as the mean percentage of injected viable E. coli). Intravascular clearance was similar in all groups. There was a significant decrease in the trapping of bacteria by the liver of CDL rats 14.5% +/- 4.95 (vs. control = 70.0% +/- 13.3) (p less than 0.005). A significant increase of bacterial trapping by the lung was observed in the CDL animals: 63.1% +/- 7.06 (vs. controls 1.4% +/- 0.82) (p less than 0.005). There was no significant change in bacterial localization in splenectomized rats. These data suggest that biliary obstruction decreases hepatic phagocytosis and increases pulmonary localization of viable E. coli. As the Kupffer cells of the liver are usually effective in removal of blood borne bacteria, this phagocytic dysfunction may contribute to the increased susceptibility to infection noted in instances of biliary obstruction. |
| Databáze: | OpenAIRE |
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