Local histamine release increases leukocyte rolling in the cerebral microcirculation of the mouse
| Autor: | Ming Qian Zheng, Tan Yong, D. Scott Linthicum |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Pathology
medicine.medical_specialty medicine.drug_class Neuroscience (miscellaneous) Inflammation Leukocyte Rolling Mice Inbred Strains Histamine Release Microcirculation Pathogenesis chemistry.chemical_compound Mice Clinical Protocols Developmental and Educational Psychology Medicine Animals Mast cell stabilizer Mast Cells Mice Inbred BALB C Venule business.industry Antibodies Monoclonal Brain Pathophysiology chemistry Histamine H2 Antagonists Regional Blood Flow Immunology Histamine H1 Antagonists Female Neurology (clinical) medicine.symptom business Cell Adhesion Molecules Histamine Blood Flow Velocity |
| Zdroj: | Brain injury. 11(10) |
| ISSN: | 0269-9052 |
| Popis: | Histamine-mediated induction of leukocyte rolling and adhesion in the cerebral microcirculation was examined in two inbred strains of mice (SJL/J and BALB/c). A cranial window was surgically prepared for the visualization of the cerebral microcirculation using intra-vital microscopy. Leukocyte rolling and adhesion to pial venular walls were assessed during off-line video playback analyses. The surgical preparation of the cranial windows was found to trigger 'spontaneous' leukocyte rolling, and this was attributed to disruption of dural mast cells and localized release of vasoactive histamine. This spontaneous leukocyte rolling was observed only in the SJL/J strain of mice, and could be prevented by presurgical treatment with the mast cell stabilizer sodium cromoglycate. BALB/c mice did not show 'spontaneous' leukocyte rolling or adhesion; this strain is known to have low numbers of CNS-associated mast cells. Exogenous histamine, applied topically to the cerebral microcirculation via the cranial window in mice pretreated with sodium cromoglycate, produced significant dose-dependent increases in leukocyte rolling and adhesion to pial venules in SJL/J mice, but not in BALB/c mice. Diphenhydramine (H1 receptor antagonist), but not cimetidine (H2 receptor antagonist), abolished both 'spontaneous' and histamine-induced leukocyte rolling. Anti-P-selectin antibody was found efficiently to block both spontaneous and histamine-induced increases in leukocyte rolling, but not leukocyte adhesion. |
| Databáze: | OpenAIRE |
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