Interaction of RECQ4 and MCM10 is important for efficient DNA replication origin firing in human cells

Autor: Lumir Krejci, Ian D. Hickson, Ganesha P. Pitchai, Maciej Kliszczak, Werner Streicher, Hana Sedlackova
Rok vydání: 2015
Předmět:
DNA Replication
Blotting
Western
Molecular Sequence Data
Apoptosis
Bone Neoplasms
Replication Origin
Saccharomyces cerevisiae
Biology
Real-Time Polymerase Chain Reaction
Origin of replication
Pre-replication complex
RecQ helicases
Chromosome Section
Immunoenzyme Techniques
Replication factor C
Minichromosome maintenance
Control of chromosome duplication
Tumor Cells
Cultured
Animals
Humans
Immunoprecipitation
Protein Interaction Domains and Motifs
Amino Acid Sequence
RNA
Messenger
Cell Proliferation
Genetics
Osteosarcoma
Minichromosome Maintenance Proteins
Sequence Homology
Amino Acid
Reverse Transcriptase Polymerase Chain Reaction
Minichromosome Maintenance Complex Component 2
Surface Plasmon Resonance
Flow Cytometry
Minichromosome Maintenance Complex Component 7
Chromatin
DNA replication origin
Research Paper: Chromosome
Licensing factor
Oncology
Origin recognition complex
Chickens
Zdroj: Oncotarget
ISSN: 1949-2553
Popis: DNA replication is a highly coordinated process that is initiated at multiple replication origins in eukaryotes. These origins are bound by the origin recognition complex (ORC), which subsequently recruits the Mcm2-7 replicative helicase in a Cdt1/Cdc6-dependent manner. In budding yeast, two essential replication factors, Sld2 and Mcm10, are then important for the activation of replication origins. In humans, the putative Sld2 homolog, RECQ4, interacts with MCM10. Here, we have identified two mutants of human RECQ4 that are deficient in binding to MCM10. We show that these RECQ4 variants are able to complement the lethality of an avian cell RECQ4 deletion mutant, indicating that the essential function of RECQ4 in vertebrates is unlikely to require binding to MCM10. Nevertheless, we show that the RECQ4-MCM10 interaction is important for efficient replication origin firing.
Databáze: OpenAIRE
Externí odkaz: