[Effects of fuzheng yiliu granules on apoptotic rate and mitochondrial membrane potential of hepatocellular carcinoma cell line H22 from mice]
| Autor: | Xue-Xi Wang, Fu-Lian Guo, De-cheng Bai, Jian-Xiong Zhao |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Male
Confocal Apoptosis Hepatic carcinoma Rhodamine 123 Flow cytometry Membrane Potentials chemistry.chemical_compound Mice Random Allocation Liver Neoplasms Experimental medicine Animals Humans Propidium iodide Membrane potential medicine.diagnostic_test Molecular biology Fluorescence Complementary and alternative medicine chemistry Mitochondrial Membranes Female Neoplasm Transplantation Drugs Chinese Herbal Phytotherapy |
| Zdroj: | Zhong xi yi jie he xue bao = Journal of Chinese integrative medicine. 4(3) |
| ISSN: | 1672-1977 |
| Popis: | OBJECTIVE To evaluate the effects of Fuzheng Yiliu Granules (FZYLG) on apoptotic rate and mitochondrial membrane potential (Delta psi m) of hepatocellular carcinoma cell line H22 from mice. METHODS Forty-eight mice inoculated with H22 cells were randomly divided into four groups: untreated group, cyclophosphamide-treated group, high-dose FZYLG-treated group and low-dose FZYLG-treated group. After 14 days of corresponding treatment, H22 cells in each group were stained with propidium iodide, and the apoptotic rates were detected by flow cytometry (FCM). The rhodamine 123 was used as a fluorescence probe to label the H22 cells, and the fluorescence intensities were observed with laser scanning confocal microscope. The fluorescence intensity of H22 cells indicated the Delta psi m of H22 cells. RESULTS FZYLG could significantly increase the apoptotic rate while reduce the Delta psi m of H22 cells from mice as compared with those in the untreated group. CONCLUSION The antitumor effects of FZYLR on H22 cells from mice are related to decreasing the Delta psi m and then inducing the apoptosis of the H22 cells. |
| Databáze: | OpenAIRE |
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