Evaluation of immature monocyte-derived dendritic cells generated from patients with colorectal cancer
| Autor: | Jacek Roliński, Sebastian Radej, Grzegorz Wallner, Sławomir Rudzki, Ryszard Maciejewski, Jacek Furmaga, Andrzej Chrościcki |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Oncology
Male medicine.medical_specialty medicine.medical_treatment Receptors Cell Surface Adenocarcinoma Immature Monocyte Immune system Immunophenotyping Antigen Reference Values Internal medicine medicine Biomarkers Tumor Humans Lectins C-Type Cells Cultured Aged Neoplasm Staging CD86 Follicular dendritic cells business.industry General Medicine Immunotherapy Dendritic Cells Middle Aged Flow Cytometry Interleukin-12 Endocytosis Cytokine Mannose-Binding Lectins Immunology Cytokines Surgery Female business Colorectal Neoplasms Cell Adhesion Molecules Mannose Receptor |
| Zdroj: | Polski przeglad chirurgiczny. 85(12) |
| ISSN: | 2299-2847 |
| Popis: | UNLABELLED Dendritic cells are heterogeneous population of the leukocytes and most potent APC in activation of naive T lymphocytes. Therefore the DCs generated in vitro are under research for their application in anti-tumor immunotherapy. The aim of the study was generation of the immature dendritic cells from peripheral blood monocytes collected from colorectal cancer patients and comparison of their ability to endocytosis, cytokine production and immunophenotype to DCs generated from healthy donors. MATERIAL AND METHODS 16 adenocarcinoma stage II patients were included in the study. Dendritic cells were generated in the presence of rhGM-CSF and IL-4. PBMC were isolated from the blood of patients and 16 healthy donors - control group. Immunophenotype, ability of endocytosis of Dextran- FITC as well as intracellular IL-12 expression of the generated dendritic cells was measured using flow cytometry. The cytokines (IL-6, IL-10, IL-12p70, IFN-γ) concentration in the supernatants of DCs culture was measured by ELISA. RESULTS The percentage of the immature dendritic cells and expression of CD206 and CD209 antigens was significantly higher in patients group (p |
| Databáze: | OpenAIRE |
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