Combination of cafeteria diet with intraperitoneally streptozotocin in rats. A type-2 diabetes model
| Autor: | Mirelly Marques Romeiro Santos, Andressa Carolina Farias Pereira Subtil Cavalcante, Luane Aparecida do Amaral, Gabriel Henrique Oliveira de Souza, Bárbara Suzuki dos Santos, Luciane Candeloro Portugal, Felipe Franscisco Bittencourt Junior, Thiago Troquez, Bruna Paola Murino Rafacho, Priscila Aiko Hiane, Elisvânia Freitas dos Santos |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Male
medicine.medical_specialty RD1-811 endocrine system diseases medicine.medical_treatment Intraperitoneal injection Cafeteria Streptozocin Diabetes Mellitus Experimental Insulin resistance Models Internal medicine Diabetes mellitus medicine Animals Rats Wistar Pancreas biology business.industry Animal Body Weight nutritional and metabolic diseases biology.organism_classification medicine.disease Streptozotocin Diet Rats Endocrinology Liver Diabetes Mellitus Type 2 Hyperglycemia Surgery Original Article medicine.symptom Steatosis business Polydipsia Weight gain medicine.drug |
| Zdroj: | Acta Cirúrgica Brasileira Acta Cirúrgica Brasileira v.36 n.7 2021 Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) instacron:SBDPC Acta Cirurgica Brasileira, Vol 36, Iss 7 (2021) |
| ISSN: | 1678-2674 0102-8650 |
| Popis: | Purpose To develop a model of induction of type-2 diabetes (DM2) by combining low doses of streptozotocin (STZ) and a cafeteria diet. Methods Forty male Wistar rats (200 g) were allocated into four groups: control (non-diabetic, n = 10); STZ 30 mg/kg (diabetic, n = 10); STZ 35 mg/kg (diabetic,n = 10); and STZ 40 mg/kg (diabetic, n = 10). DM2 was induced with a single intraperitoneal injection of STZ after four weeks of cafeteria diet in the three diabetic groups. All animals were evaluated as for anthropometric, and biochemical analyses, as well as liver, kidney and pancreas histological analyses. Results Lower weight gain, higher water intake, higher Lee index, hyperglycemia and modified total protein, urea, alpha-amylase, as well as insulin resistance, hepatic steatosis, pancreas, and kidney injury were observed in animals treated with 35 and 40 mg/kg of STZ. Conclusions The results show that the experimental model using cafeteria diet associated with 35 mg/kg of STZ is a low-cost model and efficient in order to develop DM2, confirmed by the presence of polydipsia, hyperglycemia, altered biochemical tests, insulin resistance and damages to the liver, pancreas and kidney, which is similar to the disease found in humans. |
| Databáze: | OpenAIRE |
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