Biological Screening of Eichornia crassipes against Different Pathogenic Microbes: An In Vitro Study
| Autor: | Rubina Rehman, Muhammad Shahzad Aslam, Bashir Ahmad Choudhary, Muhammad Uzair, Abdul Subhan Ijaz, Muhammad Syarhabil Ahmad |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
food.ingredient
Cytotoxicity lcsh:Medicine Aspergillus flavus Eichornia crassipes Microbiology food Bioassay Agar lcsh:QH301-705.5 Lemna biology Candida glabrata Chemistry business.industry lcsh:R General Medicine biology.organism_classification Biotechnology lcsh:Biology (General) Phytotoxicity Biological screening business Antibacterial activity Fusarium solani |
| Zdroj: | Recent Advances in Biology and Medicine, Vol 2, Iss 2016, Pp 79-82 (2016) |
| ISSN: | 2378-654X |
| Popis: | The present research is a biological screening ofEichornia crassipes(Pontederiaceae). Dichloromethane and methanol extracts of the whole plant were investigated for their antibacterial, antifungal, phytotoxic, and cytotoxic activities. The antibacterial activity was evaluated using agar well-diffusion method againstBacillus subtilis, Staphylococcus aureus, Escherichia coli, Shigella flexneri, Pseudomonas aeruginosa,andSalmonellatyphi. The antifungal activity was evaluated using the agar tube–dilution method againstCandida albicans,Candida glabrata,Aspergillus flavus,Microsporum canis,andFusarium solani. The phytotoxicity activity was determined using Lemna bioassay againstLemna minor. Brine shrimp– cytotoxicity assay was determined against brine-shrimp larvae. Dichloromethane extract exhibited significant phytotoxicity (100% growth regulation) at 1,000 μg/ml concentration againstLemna minorwhereas methanolic extracts showed moderate (75% growth regulation) phytotoxicity at the same concentration. Methanolic extract showed cytotoxicity at the highest level of dose whereas dichloromethane extract showed no activity having Etoposide as standard drug. Both of the extracts have nonsignificant antifungal and antibacterial activity. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|