Comparisons of plasma aldosterone and renin data between an automated chemiluminescent immunoanalyzer and conventional radioimmunoassays in the screening and diagnosis of primary aldosteronism
| Autor: | Chika Kyo, Takeshi Usui, Erika Watanabe, Naohisa Tamura, Tatsuhide Inoue, Eiji Nakatani, Masato Kotani, Rieko Kosugi, Tatsuo Ogawa, Rumi Shirakawa, Kanako Yamada, Hiroshi Hatakeyama, Mizuki Torii-Hanakita |
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| Rok vydání: | 2021 |
| Předmět: |
Male
Physiology Normal Distribution 030204 cardiovascular system & hematology Biochemistry Plasma renin activity law.invention Geographical Locations chemistry.chemical_compound Mathematical and Statistical Techniques 0302 clinical medicine Primary aldosteronism Japan Reference Values law Renin Blood plasma Medicine and Health Sciences Mass Screening Medicine Prospective Studies Lipid Hormones Aldosterone Multidisciplinary Statistics Radioimmunoassay Middle Aged Body Fluids Blood Physical Sciences Regression Analysis Female Anatomy Research Article Adult Asia Science Saline infusion 030209 endocrinology & metabolism Linear Regression Analysis Research and Analysis Methods Blood Plasma Veins 03 medical and health sciences Hyperaldosteronism Renin–angiotensin system Humans Statistical Methods Immunoassays Aged Chemiluminescence Health Care Policy Chromatography business.industry Biology and Life Sciences Probability Theory Probability Distribution medicine.disease Hormones Health Care Cross-Sectional Studies ROC Curve chemistry Luminescent Measurements People and Places Cardiovascular Anatomy Immunologic Techniques Blood Vessels business Mathematics Screening Guidelines |
| Zdroj: | PLoS ONE, Vol 16, Iss 7, p e0253807 (2021) PLoS ONE |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0253807 |
| Popis: | Determining values of plasma renin activity (PRA) or plasma active renin concentration (ARC), plasma aldosterone concentration (PAC), and aldosterone-to-renin ratio (ARR) is essential to diagnose primary aldosteronism (PA), but it takes several days with conventional radioimmunoassays (RIAs). Chemiluminescent enzyme immunoassays for PAC and ARC using the Accuraseed® immunoanalyzer facilitated the determination, but relations between Accuraseed® immunoanalyzer-based and RIA-based values in samples of PA confirmatory tests and adrenal venous sampling remained to be elucidated. We addressed this issue in the present study. This is a prospective, cross-sectional study. ARC and PAC values were measured by the Accuraseed® immunoanalyzer in samples, in which PRA and PAC values had been measured by the PRA-FR® RIA and SPAC®-S Aldosterone kits, respectively. The relations between Accuraseed® immunoanalyzer-based and RIA-based values were investigated with regression analyses. The optimal cutoff of Accuraseed® immunoanalyzer-based ARR for PA screening was determined by the receiver operating characteristic analysis. After log-log transformations, linear relations with high coefficients of determination were observed between Accuraseed® immunoanalyzer-based and RIA-based data of renin and aldosterone. Following the PA guidelines of Japan Endocrine Society, Accuraseed® immunoanalyzer-based cutoffs were calculated from the regression equations: the basal PAC for PA screening >12 ng/dL, PAC for the saline infusion test >8.2 ng/dL, ARC for the furosemide-upright test 3.09 ng/dL per pg/mL. The optimal cutoff of Accuraseed® immunoanalyzer-based ARR for PA screening was >2.43 ng/dL over pg/mL not to overlook bilateral PA patients. The present study provided conversion formulas between Accuraseed® immunoanalyzer-based and RIA-based values of renin, aldosterone, and ARR, not only in basal samples but also in samples of PA confirmatory tests and adrenal venous sampling. Although validation studies are awaited, the present study will become priming water of harmonization of renin and aldosterone immunoassays. |
| Databáze: | OpenAIRE |
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