Encapsulating Quantum Dots within HIV-1 Virions through Site-Specific Decoration of the Matrix Protein Enables Single Virus Tracking in Live Primary Macrophages
Autor: | Xiaowei Zhang, Cui Zongqiang, Zhiping Zhang, Wei Li, Qin Li, Xian-En Zhang, Wen Yin |
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Rok vydání: | 2018 |
Předmět: |
Models
Molecular 0301 basic medicine viruses HIV Infections Bioengineering Peptide 02 engineering and technology Matrix (biology) Mitochondrial Dynamics Virus Cell Line Viral Matrix Proteins 03 medical and health sciences chemistry.chemical_compound Biotin Viral entry Quantum Dots Humans Biotinylation General Materials Science Cells Cultured chemistry.chemical_classification Viral matrix protein Staining and Labeling Macrophages Mechanical Engineering Optical Imaging Virion technology industry and agriculture General Chemistry Virus Internalization 021001 nanoscience & nanotechnology Condensed Matter Physics Mitochondria Cell biology 030104 developmental biology Microscopy Fluorescence chemistry Quantum dot HIV-1 0210 nano-technology |
Zdroj: | Nano Letters. 18:7457-7468 |
ISSN: | 1530-6992 1530-6984 |
Popis: | Labeling and imaging with quantum dots (QDs) provides powerful tools to visualize viral infection in living cells. Encapsulating QDs within virions represents a novel strategy for virus labeling. Here, we developed infectious HIV-1 virions encapsulating QDs through site-specific decoration of the viral matrix protein (MA) and used them to visualize early infection events in human primary macrophages by single-particle imaging. The MA protein was fused to a biotin acceptor peptide (BAP) tag, biotinylated, complexed with streptavidin-conjugated QDs in live cells, and incorporated into virions during virus assembly. The QD-encapsulated virions were tracked during infection of macrophages at a single particle level. The dynamic dissociation of MA and Vpr was also tracked in real time, and the results demonstrated that MA has multiple dynamic behaviors and functions during virus entry. More importantly, we tracked the dynamic interplay of QD-encapsulated virions with cellular mitochondria in live primary macrophages. We also found that HIV-1 can induce fission of mitochondria during the early phases of infection. In summary, we have constructed a type of QD-encapsulated virus particle and used this technology to further our understanding of the early events of HIV-1 infection. |
Databáze: | OpenAIRE |
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