Two highly validated multiplexes (12‐plex and 8‐plex) for species delimitation and parentage analysis in oaks (Quercus spp.)
| Autor: | Stefanie Wagner, P. Léger, Rémy J. Petit, Erwan Guichoux, L. Lagache |
|---|---|
| Přispěvatelé: | Biodiversité, Gènes & Communautés (BioGeCo), Institut National de la Recherche Agronomique (INRA)-Université de Bordeaux (UB), Centre de recherche Pernod Ricard, Steinmann Institut, Rheinische Friedrich-Wilhelms-Universität Bonn, Research Center of Pernod-Ricard (CRPR), EVOLTREE Network of Excellence, European Commission [016322], Eranet Biodiversa programme [ANR-08-BDVA-006] |
| Rok vydání: | 2011 |
| Předmět: |
0106 biological sciences
Genotype [SDV]Life Sciences [q-bio] QUERCUS ROBUR MICROSATELLITES MULTIPLEXE Computational biology CHENE ROUVRE Polymerase Chain Reaction 010603 evolutionary biology 01 natural sciences CHENE Quercus CHENE PEDONCULE 03 medical and health sciences Multiplex polymerase chain reaction QUERCUS SESSILIFLORA Genetics Humans Multiplex Allele Genotyping Alleles Ecology Evolution Behavior and Systematics MANUAL BINNING 030304 developmental biology 0303 health sciences biology food and beverages Genetic data ISOLATION DE L'ADN biology.organism_classification QUERCUS SPP QUERCUS PETRAEA Quercus spp Microsatellite MOLECULAR IDENTIFICATION Quercus petraea Microsatellite Repeats Biotechnology |
| Zdroj: | Molecular Ecology Resources Molecular Ecology Resources, Wiley/Blackwell, 2011, 11 (3), pp.578-585. ⟨10.1111/j.1755-0998.2011.02983.x⟩ |
| ISSN: | 1755-0998 1755-098X |
| DOI: | 10.1111/j.1755-0998.2011.02983.x |
| Popis: | International audience; Multiplex PCR is a fast and cost-effective technique allowing increased genotyping throughput of microsatellites. We developed two multiplexes for Quercus petraea and Q. robur, a 12-plex of EST-SSRs (eSSRs) and an 8-plex of genomic SSRs (gSSRs). We studied the origin of allele calling errors at the human reader and software levels. We showed that the robustness of allele identification can be improved by binning on raw peak sizes prior to genetic data analysis. We checked through simulation the power of these markers for species delimitation and hybrid detection. The resolution achieved with all 20 markers was greatly improved compared to that of previous studies based on a subset of the markers. Preliminary PCR tests suggest that these multiplexes might be useful to study other oak species as well. The strategy used for multiplex microsatellite development (from PCR conditions to the definition of allele calling rules) should be broadly applicable. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text