Examining acute and chronic effects of short- and long-chain fatty acids on peptide YY (PYY) gene expression, cellular storage and secretion in STC-1 cells
| Autor: | Christine M. Bruen, Fiona O'Halloran, Danielle Calderwood, Brian D. Green, Harsh Panwar, Linda Giblin, Katharine V. Hand |
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| Rok vydání: | 2012 |
| Předmět: |
DNA Replication
medicine.medical_specialty Cell Survival media_common.quotation_subject Medicine (miscellaneous) Enteroendocrine cell Gastric Inhibitory Polypeptide Fatty Acids Nonesterified Cell Line Linoleic Acid Mice Cellular storage Glucagon-Like Peptide 1 Internal medicine Gene expression medicine Animals Linoleic Acids Conjugated Peptide YY Secretion RNA Messenger Pentanoic Acids media_common Secretory Pathway Nutrition and Dietetics Chemistry digestive oral and skin physiology Appetite Fatty Acids Volatile Up-Regulation Kinetics Enterocytes Endocrinology Gastrointestinal hormone Cholecystokinin hormones hormone substitutes and hormone antagonists Homeostasis |
| Zdroj: | European Journal of Nutrition. 52:1303-1313 |
| ISSN: | 1436-6215 1436-6207 |
| DOI: | 10.1007/s00394-012-0439-9 |
| Popis: | Peptide YY (PYY) is a gastrointestinal hormone with physiological actions regulating appetite and energy homoeostasis. The cellular mechanisms by which nutrients stimulate PYY secretion from intestinal enteroendocrine cells are still being elucidated.This study comprehensively evaluated the suitability of intestinal STC-1 cells as an in vitro model of PYY secretion. PYY concentrations (both intracellular and in culture media) with other intestinal peptides (CCK, GLP-1 and GIP) demonstrated that PYY is a prominent product of STC-1 cells. Furthermore, acute and chronic PYY responses to 15 short (SCFAs)- and long-chain (LCFAs) dietary fatty acids were measured alongside parameters for DNA synthesis, cell viability and cytotoxicity.We found STC-1 cells to be reliable secretors of PYY constitutively releasing PYY into cell culture media (but not into non-stimulatory buffer). We demonstrate for the first time that STC-1 cells produce PYY mRNA transcripts; that STC-1 cells produce specific time- and concentration-dependent PYY secretory responses to valeric acid; that linoleic acid and conjugated linoleic acid 9,11 (CLA 9,11) are potent PYY secretagogues; and that chronic exposure of SCFAs and LCFAs can be detrimental to STC-1 cells.Our studies demonstrate the potential usefulness of STC-1 cells as an in vitro model for investigating nutrient-stimulated PYY secretion in an acute setting. Furthermore, our discovery that CLA directly stimulates L-cells to secrete PYY indicates another possible mechanism contributing to the observed effects of dietary CLA on weight loss. |
| Databáze: | OpenAIRE |
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